The interaction of monocytes with rheumatoid synovial cells is a key step in LIGHT-mediated inflammatory bone destruction

Satoru Ishida, Shoji Yamane, Saori Nakano, Toru Yanagimoto, Yukie Hanamoto, Miki Maeda-Tanimura, Tomoko Toyosaki-Maeda, Jun Ishizaki, Yoshiyuki Matsuo, Naoshi Fukui, Tsunetoshi Itoh, Takahiro Ochi, Ryuji Suzuki

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Formation of osteoclasts and consequent joint destruction are hallmarks of rheumatoid arthritis (RA). Here we show that LIGHT, a member of the tumour necrosis factor (TNF) superfamily, induced the differentiation into tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs) of CD14+ monocytes cocultured with nurse-like cells isolated from RA synovium, but not of freshly isolated CD14+ monocytes. Receptor activator of nuclear factor-κB ligand (RANKL) enhanced this LIGHT-induced generation of TRAP-positive MNCs. The MNCs showed the phenotypical and functional characteristics of osteoclasts; they showed the expression of osteoclast markers such as cathepsin K, actin-ring formation, and the ability to resorb bone. Moreover, the MNCs expressed both matrix metalloproteinase 9 (MMP-9) and MMP-12, but the latter was not expressed in osteoclasts induced from CD14+ monocytes by RANKL. Immunohistochemical analysis showed that the MMP-12-producing MNCs were present in the erosive areas of joints in RA, but not in the affected joints of osteoarthritic patients. These findings suggested that LIGHT might be involved in the progression of inflammatory bone destruction in RA, and that osteoclast progenitors might become competent for LIGHT-mediated osteoclastogenesis via interactions with synoviocyte-like nurse-like cells.

Original languageEnglish
Pages (from-to)e315-e324
Issue number1 PART 2
Publication statusPublished - 2009 Sept
Externally publishedYes


  • Differentiation
  • Monocyte
  • Osteoclast
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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