TY - JOUR
T1 - The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis
AU - Saito, Hikaru
AU - Oikawa, Takaya
AU - Hamamoto, Shin
AU - Ishimaru, Yasuhiro
AU - Kanamori-Sato, Miyu
AU - Sasaki-Sekimoto, Yuko
AU - Utsumi, Tomoya
AU - Chen, Jing
AU - Kanno, Yuri
AU - Masuda, Shinji
AU - Kamiya, Yuji
AU - Seo, Mitsunori
AU - Uozumi, Nobuyuki
AU - Ueda, Minoru
AU - Ohta, Hiroyuki
N1 - Funding Information:
We thank E. E. Farmer for critical reading of our manuscript. We also thank M. Shi-mojima, K. Hori and other Ohta & Masuda lab members for critical comments and/or technical help. Full-length cDNAs were provided by RIKEN Bio Resource Center (BRC). This work was supported in part by the Global COE Program (From the Earth to ‘Earths’) for H.S. and H.O., a Grant-in-Aid for Scientific Research (No.24580135 and 26102711 to S.H, 25119725 to M.S. and 25292055 to N.U.), the Botanical Research Grant by the New Technology Development Foundation for Y.I. and a Grant-in-Aid for Scientific Research (No.23102012) on Innovative Areas Chemical Biology of Natural Products" (No.2301) for M.U. from MEXT, Japan. Hormonome analysis was supported by the Japan Advanced Plant Science Network.
Publisher Copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.
PY - 2015/2/4
Y1 - 2015/2/4
N2 - Plant hormones are transported across cell membranes during various physiological events. Recent identification of abscisic acid and strigolactone transporters suggests that transport of various plant hormones across membranes does not occur by simple diffusion but requires transporter proteins that are strictly regulated during development. Here, we report that a major glucosinolate transporter, GTR1/NPF2.10, is multifunctional and may be involved in hormone transport in Arabidopsis thaliana. When heterologously expressed in oocytes, GTR1 transports jasmonoyl-isoleucine and gibberellin in addition to glucosinolates. gtr1 mutants are severely impaired in filament elongation and anther dehiscence resulting in reduced fertility, but these phenotypes can be rescued by gibberellin treatment. These results suggest that GTR1 may be a multifunctional transporter for the structurally distinct compounds glucosinolates, jasmonoyl-isoleucine and gibberellin, and may positively regulate stamen development by mediating gibberellin supply.
AB - Plant hormones are transported across cell membranes during various physiological events. Recent identification of abscisic acid and strigolactone transporters suggests that transport of various plant hormones across membranes does not occur by simple diffusion but requires transporter proteins that are strictly regulated during development. Here, we report that a major glucosinolate transporter, GTR1/NPF2.10, is multifunctional and may be involved in hormone transport in Arabidopsis thaliana. When heterologously expressed in oocytes, GTR1 transports jasmonoyl-isoleucine and gibberellin in addition to glucosinolates. gtr1 mutants are severely impaired in filament elongation and anther dehiscence resulting in reduced fertility, but these phenotypes can be rescued by gibberellin treatment. These results suggest that GTR1 may be a multifunctional transporter for the structurally distinct compounds glucosinolates, jasmonoyl-isoleucine and gibberellin, and may positively regulate stamen development by mediating gibberellin supply.
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U2 - 10.1038/ncomms7095
DO - 10.1038/ncomms7095
M3 - Article
C2 - 25648767
AN - SCOPUS:84929110780
SN - 2041-1723
VL - 6
JO - Nature Communications
JF - Nature Communications
M1 - 6095
ER -