The mRNA distribution of C7orf24, a γ-glutamyl cyclotransferase, in rat tissues

Keisuke Oda; Satoshi Makino; Chiaki Masuda; Tatsuhiro Yoshiki; Yoshihisa Kitamura; Kazuyuki Takata; Daijiro Yanagisawa; Takashi Taniguchi; Ikuo Tooyama

doi:10.1369/jhc.2009.953976

The mRNA distribution of C7orf24, a γ-glutamyl cyclotransferase, in rat tissues

Keisuke Oda, Satoshi Makino, Chiaki Masuda, Tatsuhiro Yoshiki, Yoshihisa Kitamura, Kazuyuki Takata, Daijiro Yanagisawa, Takashi Taniguchi, Ikuo Tooyama

Tohoku Medical Megabank Organization (ToMMo)

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The putative protein C7orf24 is encoded by Homo sapiens chromosome 7 open reading frame 24. C7orf24 was first identified as a 21-kDa cytochrome c-releasing factor detected in the cytosolic fraction of human leukemia U937 cells after treatment with geranylgeraniol. C7orf24 protein was recently identified as a γ-glutamyl cyclotransferase, an enzyme in the γ-glutamyl cycle. However, the exact localization of C7orf24 mRNA in normal tissues remains unknown. The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. The RT-PCR experiments demonstrated that C7orf24 and a variant C7orf24 mRNA were expressed in various tissues. Quantitative RT-PCR analysis revealed significantly high levels of both C7orf24 mRNAs in the liver and kidney, compared with other tissues examined. In situ hybridization histochemistry localized C7orf24mRNA to hepatocytes in the liver and renal tubules in the kidney. The present results thus implicated an important role for C7orf24 in liver and kidney. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

Original language	English
Pages (from-to)	1121-1126
Number of pages	6
Journal	Journal of Histochemistry and Cytochemistry
Volume	57
Issue number	12
DOIs	https://doi.org/10.1369/jhc.2009.953976
Publication status	Published - 2009 Dec

Keywords

Detoxification
Drug metabolism
Glutathione
In situ hybridization

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10.1369/jhc.2009.953976

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title = "The mRNA distribution of C7orf24, a γ-glutamyl cyclotransferase, in rat tissues",

abstract = "The putative protein C7orf24 is encoded by Homo sapiens chromosome 7 open reading frame 24. C7orf24 was first identified as a 21-kDa cytochrome c-releasing factor detected in the cytosolic fraction of human leukemia U937 cells after treatment with geranylgeraniol. C7orf24 protein was recently identified as a γ-glutamyl cyclotransferase, an enzyme in the γ-glutamyl cycle. However, the exact localization of C7orf24 mRNA in normal tissues remains unknown. The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. The RT-PCR experiments demonstrated that C7orf24 and a variant C7orf24 mRNA were expressed in various tissues. Quantitative RT-PCR analysis revealed significantly high levels of both C7orf24 mRNAs in the liver and kidney, compared with other tissues examined. In situ hybridization histochemistry localized C7orf24mRNA to hepatocytes in the liver and renal tubules in the kidney. The present results thus implicated an important role for C7orf24 in liver and kidney. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.",

keywords = "Detoxification, Drug metabolism, Glutathione, In situ hybridization",

author = "Keisuke Oda and Satoshi Makino and Chiaki Masuda and Tatsuhiro Yoshiki and Yoshihisa Kitamura and Kazuyuki Takata and Daijiro Yanagisawa and Takashi Taniguchi and Ikuo Tooyama",

year = "2009",

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doi = "10.1369/jhc.2009.953976",

language = "English",

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AU - Oda, Keisuke

AU - Makino, Satoshi

AU - Masuda, Chiaki

AU - Yoshiki, Tatsuhiro

AU - Kitamura, Yoshihisa

AU - Takata, Kazuyuki

AU - Yanagisawa, Daijiro

AU - Taniguchi, Takashi

AU - Tooyama, Ikuo

PY - 2009/12

Y1 - 2009/12

N2 - The putative protein C7orf24 is encoded by Homo sapiens chromosome 7 open reading frame 24. C7orf24 was first identified as a 21-kDa cytochrome c-releasing factor detected in the cytosolic fraction of human leukemia U937 cells after treatment with geranylgeraniol. C7orf24 protein was recently identified as a γ-glutamyl cyclotransferase, an enzyme in the γ-glutamyl cycle. However, the exact localization of C7orf24 mRNA in normal tissues remains unknown. The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. The RT-PCR experiments demonstrated that C7orf24 and a variant C7orf24 mRNA were expressed in various tissues. Quantitative RT-PCR analysis revealed significantly high levels of both C7orf24 mRNAs in the liver and kidney, compared with other tissues examined. In situ hybridization histochemistry localized C7orf24mRNA to hepatocytes in the liver and renal tubules in the kidney. The present results thus implicated an important role for C7orf24 in liver and kidney. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

AB - The putative protein C7orf24 is encoded by Homo sapiens chromosome 7 open reading frame 24. C7orf24 was first identified as a 21-kDa cytochrome c-releasing factor detected in the cytosolic fraction of human leukemia U937 cells after treatment with geranylgeraniol. C7orf24 protein was recently identified as a γ-glutamyl cyclotransferase, an enzyme in the γ-glutamyl cycle. However, the exact localization of C7orf24 mRNA in normal tissues remains unknown. The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. The RT-PCR experiments demonstrated that C7orf24 and a variant C7orf24 mRNA were expressed in various tissues. Quantitative RT-PCR analysis revealed significantly high levels of both C7orf24 mRNAs in the liver and kidney, compared with other tissues examined. In situ hybridization histochemistry localized C7orf24mRNA to hepatocytes in the liver and renal tubules in the kidney. The present results thus implicated an important role for C7orf24 in liver and kidney. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

KW - Detoxification

KW - Drug metabolism

KW - Glutathione

KW - In situ hybridization

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The mRNA distribution of C7orf24, a γ-glutamyl cyclotransferase, in rat tissues

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Keywords

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