The Oxygen Gradient in Hypoxic Conditions Enhances and Guides Dictyostelium discoideum Migration

Satomi Hirose, Jean Paul Rieu, Olivier Cochet-Escartin, Christophe Anjard, Kenichi Funamoto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Spatiotemporal variations of oxygen concentration affect the cell behaviors that are involved in physiological and pathological events. In our previous study with Dictyostelium discoideum (Dd) as a model of cell motility, aggregations of Dd cells exhibited long-lasting and highly stable migration in a self-generated hypoxic environment, forming a ring shape that spread toward the outer higher oxygen region. However, it is still unclear what kinds of changes in the migratory properties are responsible for the observed phenomena. Here, we investigated the migration of Dd to clarify the oxygen-dependent characteristics of aerokinesis and aerotaxis. Migratory behaviors of Dd cells were analyzed under various oxygen concentration gradients and uniform oxygen conditions generated in microfluidic devices. Under hypoxic conditions below 2% O2, corresponding to less than 25 µM O2 in the culture medium, the migration of Dd cells was enhanced (aerokinesis) and the oxygen gradient guided the cells toward the oxygen-rich region (aerotaxis). The aerotaxis was attributed to the increase in the frequency of migration associated with the direction of higher O2, the acceleration of migration velocity, and the enhancement of migration straightness. Thus, aero-kinesis and aerotaxis are dependent on both the oxygen level and possibly relative gradient and are essential mechanisms for the migration of Dd.

Original languageEnglish
Article number318
Issue number2
Publication statusPublished - 2022 Feb


  • Aerotaxis
  • Cell migration
  • Dictyostelium discoideum
  • Microfluidic device
  • Oxygen

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Process Chemistry and Technology


Dive into the research topics of 'The Oxygen Gradient in Hypoxic Conditions Enhances and Guides Dictyostelium discoideum Migration'. Together they form a unique fingerprint.

Cite this