TY - JOUR
T1 - The Oxygen Gradient in Hypoxic Conditions Enhances and Guides Dictyostelium discoideum Migration
AU - Hirose, Satomi
AU - Rieu, Jean Paul
AU - Cochet-Escartin, Olivier
AU - Anjard, Christophe
AU - Funamoto, Kenichi
N1 - Funding Information:
Funding: This research was funded by the JSPS Overseas Challenge Program for Young Researchers (to S.H.), by JST SPRING, Grant Number JPMJSP2114 (to S.H.), by the Collaborative Research Project of the Institute of Fluid Science, Tohoku University, Grant Number J21Ly04 (to J.-P.R and K.F.), by the CNRS—MITI—Défi Modélisation du vivant—2019 (to J.-P.R) and by the International Human Frontier Science Program Organization, Grant Number RGP0051/2021 (to J.-P.R).
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2
Y1 - 2022/2
N2 - Spatiotemporal variations of oxygen concentration affect the cell behaviors that are involved in physiological and pathological events. In our previous study with Dictyostelium discoideum (Dd) as a model of cell motility, aggregations of Dd cells exhibited long-lasting and highly stable migration in a self-generated hypoxic environment, forming a ring shape that spread toward the outer higher oxygen region. However, it is still unclear what kinds of changes in the migratory properties are responsible for the observed phenomena. Here, we investigated the migration of Dd to clarify the oxygen-dependent characteristics of aerokinesis and aerotaxis. Migratory behaviors of Dd cells were analyzed under various oxygen concentration gradients and uniform oxygen conditions generated in microfluidic devices. Under hypoxic conditions below 2% O2, corresponding to less than 25 µM O2 in the culture medium, the migration of Dd cells was enhanced (aerokinesis) and the oxygen gradient guided the cells toward the oxygen-rich region (aerotaxis). The aerotaxis was attributed to the increase in the frequency of migration associated with the direction of higher O2, the acceleration of migration velocity, and the enhancement of migration straightness. Thus, aero-kinesis and aerotaxis are dependent on both the oxygen level and possibly relative gradient and are essential mechanisms for the migration of Dd.
AB - Spatiotemporal variations of oxygen concentration affect the cell behaviors that are involved in physiological and pathological events. In our previous study with Dictyostelium discoideum (Dd) as a model of cell motility, aggregations of Dd cells exhibited long-lasting and highly stable migration in a self-generated hypoxic environment, forming a ring shape that spread toward the outer higher oxygen region. However, it is still unclear what kinds of changes in the migratory properties are responsible for the observed phenomena. Here, we investigated the migration of Dd to clarify the oxygen-dependent characteristics of aerokinesis and aerotaxis. Migratory behaviors of Dd cells were analyzed under various oxygen concentration gradients and uniform oxygen conditions generated in microfluidic devices. Under hypoxic conditions below 2% O2, corresponding to less than 25 µM O2 in the culture medium, the migration of Dd cells was enhanced (aerokinesis) and the oxygen gradient guided the cells toward the oxygen-rich region (aerotaxis). The aerotaxis was attributed to the increase in the frequency of migration associated with the direction of higher O2, the acceleration of migration velocity, and the enhancement of migration straightness. Thus, aero-kinesis and aerotaxis are dependent on both the oxygen level and possibly relative gradient and are essential mechanisms for the migration of Dd.
KW - Aerotaxis
KW - Cell migration
KW - Dictyostelium discoideum
KW - Microfluidic device
KW - Oxygen
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U2 - 10.3390/pr10020318
DO - 10.3390/pr10020318
M3 - Article
AN - SCOPUS:85124426435
SN - 2227-9717
VL - 10
JO - Processes
JF - Processes
IS - 2
M1 - 318
ER -