The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine

G. H.M. Sagor; Masataka Inoue; Dong Wook Kim; Seiji Kojima; Masaru Niitsu; Thomas Berberich; Tomonobu Kusano

doi:10.1016/j.febslet.2015.08.045

The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine

G. H.M. Sagor, Masataka Inoue, Dong Wook Kim, Seiji Kojima, Masaru Niitsu, Thomas Berberich, Tomonobu Kusano

Creative Interdisciplinary Research Division

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15 Citations (Scopus)

Abstract

In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.

Original language	English
Article number	37356
Pages (from-to)	3071-3078
Number of pages	8
Journal	FEBS Letters
Volume	589
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2015.08.045
Publication status	Published - 2015 Oct 7

Keywords

Norspermidine
Polyamine oxidase
Selaginella lepidophylla
Spermidine
Spermine
Thermospermine

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2015.08.045

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title = "The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine",

abstract = "In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.",

keywords = "Norspermidine, Polyamine oxidase, Selaginella lepidophylla, Spermidine, Spermine, Thermospermine",

author = "Sagor, {G. H.M.} and Masataka Inoue and Kim, {Dong Wook} and Seiji Kojima and Masaru Niitsu and Thomas Berberich and Tomonobu Kusano",

note = "Funding Information: GHMS is supported by the JSPS postdoctoral fellowship for foreign researchers. Funding Information: We thank Dr. Yoshiya Seto (Tohoku University) for help with the LC–MS/MS analysis. Mr. Kiyoshi Ishikawa is acknowledged for his guidance in cultivating Selaginella plants. This study is supported by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) to TK ( 26·04081 , 15K14705 ) and to DWK ( 25·5682 ). Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies",

year = "2015",

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doi = "10.1016/j.febslet.2015.08.045",

language = "English",

volume = "589",

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journal = "FEBS Letters",

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T1 - The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine

AU - Sagor, G. H.M.

AU - Inoue, Masataka

AU - Kim, Dong Wook

AU - Kojima, Seiji

AU - Niitsu, Masaru

AU - Berberich, Thomas

AU - Kusano, Tomonobu

N1 - Funding Information: GHMS is supported by the JSPS postdoctoral fellowship for foreign researchers. Funding Information: We thank Dr. Yoshiya Seto (Tohoku University) for help with the LC–MS/MS analysis. Mr. Kiyoshi Ishikawa is acknowledged for his guidance in cultivating Selaginella plants. This study is supported by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) to TK ( 26·04081 , 15K14705 ) and to DWK ( 25·5682 ). Publisher Copyright: © 2015 Federation of European Biochemical Societies

PY - 2015/10/7

Y1 - 2015/10/7

N2 - In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.

AB - In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.

KW - Norspermidine

KW - Polyamine oxidase

KW - Selaginella lepidophylla

KW - Spermidine

KW - Spermine

KW - Thermospermine

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The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine

Abstract

Keywords

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