TY - JOUR
T1 - The protective effect of pyrroloquinoline quinone and its derivatives against carbon tetrachloride‐induced liver injury of rats
AU - TSUCHIDA, TOSHIHIRO
AU - YASUYAMA, TOSHIFUMI
AU - HIGUCHI, KIYOHIRO
AU - WATANABE, AKIHARU
AU - URAKAMI, TEIJI
AU - AKAIKE, TAKAAKI
AU - SATO, KEIZO
AU - MAEDA, HIROSHI
PY - 1993/8
Y1 - 1993/8
N2 - Pyrroloquinoline quinone (PQQ) and its derivative, oxazo pyrroloquinoline (OPQ‐G), protected rats from experimental liver injury induced by carbon tetrachloride (CCl4) in vivo. This effect was observed after an intraperitoneal injection of 5 mg/kg PQQ or OPQ‐G, which was given twice, 10 min and 1 h before CCl4 administration. Pyrroloquinoline quinone protected primary cultured rat hepatocytes from CCl4 toxicity in vitro. This protection was most effective at a concentration of 3 μmol/L PQQ. Pyrroloquinoline quinone derivatives (oxazo pyrroloquinoline, methyl‐thioethyl oxazo pyrroloquinoline and PQQ‐allylester) also protected the hepatocytes from CCl4 toxicity. Pyrroloquinoline quinone and its derivatives inhibited the lucigenin‐enhanced chemiluminescence from isolated hepatocytes initiated by CCl4. These results suggest that eliminating free radicals is one of the protective mechanisms of PQQ and its derivatives against CCl4‐induced liver injury.
AB - Pyrroloquinoline quinone (PQQ) and its derivative, oxazo pyrroloquinoline (OPQ‐G), protected rats from experimental liver injury induced by carbon tetrachloride (CCl4) in vivo. This effect was observed after an intraperitoneal injection of 5 mg/kg PQQ or OPQ‐G, which was given twice, 10 min and 1 h before CCl4 administration. Pyrroloquinoline quinone protected primary cultured rat hepatocytes from CCl4 toxicity in vitro. This protection was most effective at a concentration of 3 μmol/L PQQ. Pyrroloquinoline quinone derivatives (oxazo pyrroloquinoline, methyl‐thioethyl oxazo pyrroloquinoline and PQQ‐allylester) also protected the hepatocytes from CCl4 toxicity. Pyrroloquinoline quinone and its derivatives inhibited the lucigenin‐enhanced chemiluminescence from isolated hepatocytes initiated by CCl4. These results suggest that eliminating free radicals is one of the protective mechanisms of PQQ and its derivatives against CCl4‐induced liver injury.
KW - carbon tetrachloride liver injury
KW - chemiluminescence
KW - free radical
KW - lucigenin
KW - oxazo pyrroloquinoline
KW - primary cultured hepatocyte
KW - pyrroloquinoline quinone
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U2 - 10.1111/j.1440-1746.1993.tb01525.x
DO - 10.1111/j.1440-1746.1993.tb01525.x
M3 - Article
C2 - 8397011
AN - SCOPUS:0027198867
SN - 0815-9319
VL - 8
SP - 342
EP - 347
JO - Journal of Gastroenterology and Hepatology (Australia)
JF - Journal of Gastroenterology and Hepatology (Australia)
IS - 4
ER -