The rice mitochondrial iron transporter is essential for plant growth

Khurram Bashir; Yasuhiro Ishimaru; Hugo Shimo; Seiji Nagasaka; Masaru Fujimoto; Hideki Takanashi; Nobuhiro Tsutsumi; Gynheung An; Hiromi Nakanishi; Naoko K. Nishizawa

doi:10.1038/ncomms1326

The rice mitochondrial iron transporter is essential for plant growth

Khurram Bashir, Yasuhiro Ishimaru, Hugo Shimo, Seiji Nagasaka, Masaru Fujimoto, Hideki Takanashi, Nobuhiro Tsutsumi, Gynheung An, Hiromi Nakanishi, Naoko K. Nishizawa

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

123 Citations (Scopus)

Abstract

In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Δmrs3Δmrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.

Original language	English
Article number	322
Journal	Nature communications
Volume	2
Issue number	1
DOIs	https://doi.org/10.1038/ncomms1326
Publication status	Published - 2011

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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title = "The rice mitochondrial iron transporter is essential for plant growth",

abstract = "In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Δmrs3Δmrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.",

author = "Khurram Bashir and Yasuhiro Ishimaru and Hugo Shimo and Seiji Nagasaka and Masaru Fujimoto and Hideki Takanashi and Nobuhiro Tsutsumi and Gynheung An and Hiromi Nakanishi and Nishizawa, {Naoko K.}",

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AU - Bashir, Khurram

AU - Ishimaru, Yasuhiro

AU - Shimo, Hugo

AU - Nagasaka, Seiji

AU - Fujimoto, Masaru

AU - Takanashi, Hideki

AU - Tsutsumi, Nobuhiro

AU - An, Gynheung

AU - Nakanishi, Hiromi

AU - Nishizawa, Naoko K.

PY - 2011

Y1 - 2011

N2 - In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Δmrs3Δmrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.

AB - In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Δmrs3Δmrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.

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The rice mitochondrial iron transporter is essential for plant growth

Abstract

ASJC Scopus subject areas

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