TY - JOUR
T1 - The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function
AU - Ugajin, Tomohisa
AU - Terada, Yukihiro
AU - Hasegawa, Hisataka
AU - Nabeshima, Hiroshi
AU - Suzuki, Kichiya
AU - Yaegashi, Nobuo
N1 - Funding Information:
Acknowledgements This study was supported by Japan Society for the Promotion of Science to Y.T., and N.Y.
PY - 2010/2
Y1 - 2010/2
N2 - Purpose: To evaluate whether the morphology of the sperm midpiece observed by high magnification microscopy relates to sperm centrosomal function. Methods: Sperm selected by conventional microscopy were defined as controls. By high magnification microscopy, sperm with straight midpieces were defined as Group 1, while those with tapering midpieces were defined as Group 2. Heterologous ICSI of human sperm into bovine oocytes was used to assess human sperm centrosomal function and analysis of sperm aster formation. Results: The total rate of sperm aster formation was 80.5% in Group 1, which was significantly higher (P∈<∈0.05) than the rate of 33.3% seen for Group 2. Furthermore, sperm aster formation rates tended to be higher for Group 1 than for the controls. Conclusions: This study demonstrates improvement of sperm aster formation rates by selecting sperm on the basis of midpiece morphology. The injection of selected sperm bearing morphologically straight midpieces may contribute to improved expression of sperm centrosomal function, providing a positive effect on fertilization after ICSI.
AB - Purpose: To evaluate whether the morphology of the sperm midpiece observed by high magnification microscopy relates to sperm centrosomal function. Methods: Sperm selected by conventional microscopy were defined as controls. By high magnification microscopy, sperm with straight midpieces were defined as Group 1, while those with tapering midpieces were defined as Group 2. Heterologous ICSI of human sperm into bovine oocytes was used to assess human sperm centrosomal function and analysis of sperm aster formation. Results: The total rate of sperm aster formation was 80.5% in Group 1, which was significantly higher (P∈<∈0.05) than the rate of 33.3% seen for Group 2. Furthermore, sperm aster formation rates tended to be higher for Group 1 than for the controls. Conclusions: This study demonstrates improvement of sperm aster formation rates by selecting sperm on the basis of midpiece morphology. The injection of selected sperm bearing morphologically straight midpieces may contribute to improved expression of sperm centrosomal function, providing a positive effect on fertilization after ICSI.
KW - Centrosomal function
KW - Intracytoplasmic morphologically selected sperm injection (IMSI)
KW - Sperm aster formation
KW - Sperm midpiece
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U2 - 10.1007/s10815-009-9371-1
DO - 10.1007/s10815-009-9371-1
M3 - Article
C2 - 20012684
AN - SCOPUS:77951248304
SN - 1058-0468
VL - 27
SP - 75
EP - 81
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 2-3
ER -