The sodium channel of human excitable cells is a target for gambierol

M. Carmen Louzao; Eva Cagide; Mercedes R. Vieytes; Makoto Sasaki; Haruhiko Fuwa; Takeshi Yasumoto; Luis M. Botana

doi:10.1159/000094138

The sodium channel of human excitable cells is a target for gambierol

M. Carmen Louzao, Eva Cagide, Mercedes R. Vieytes, Makoto Sasaki, Haruhiko Fuwa, Takeshi Yasumoto, Luis M. Botana

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Background: Gambierol is a polycyclic ether toxin with the same biogenetic origin as ciguatoxins. Gambierol has been associated with neurological symptoms in humans even though its mechanism of action has not been fully characterized. Methods: We studied the effect of gambierol in human neuroblastoma cells by using bis-oxonol to measure membrane potential and FURA-2 to monitor intracellular calcium. Results: We found that this toxin: i) produced a membrane depolarization, ii) potentiated the effect of veratridine on membrane potential iii) decreased ciguatoxin-induced depolarization and iv) increased cytosolic calcium in neuroblastoma cells. Conclusion: These results indicate that gambierol modulate ion fluxes by acting as a partial agonist of sodium channels.

Original language	English
Pages (from-to)	257-268
Number of pages	12
Journal	Cellular Physiology and Biochemistry
Volume	17
Issue number	5-6
DOIs	https://doi.org/10.1159/000094138
Publication status	Published - 2006

Keywords

Gambierol
Neuroblastoma
Sodium channel

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10.1159/000094138

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title = "The sodium channel of human excitable cells is a target for gambierol",

abstract = "Background: Gambierol is a polycyclic ether toxin with the same biogenetic origin as ciguatoxins. Gambierol has been associated with neurological symptoms in humans even though its mechanism of action has not been fully characterized. Methods: We studied the effect of gambierol in human neuroblastoma cells by using bis-oxonol to measure membrane potential and FURA-2 to monitor intracellular calcium. Results: We found that this toxin: i) produced a membrane depolarization, ii) potentiated the effect of veratridine on membrane potential iii) decreased ciguatoxin-induced depolarization and iv) increased cytosolic calcium in neuroblastoma cells. Conclusion: These results indicate that gambierol modulate ion fluxes by acting as a partial agonist of sodium channels.",

keywords = "Gambierol, Neuroblastoma, Sodium channel",

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T1 - The sodium channel of human excitable cells is a target for gambierol

AU - Louzao, M. Carmen

AU - Cagide, Eva

AU - Vieytes, Mercedes R.

AU - Sasaki, Makoto

AU - Fuwa, Haruhiko

AU - Yasumoto, Takeshi

AU - Botana, Luis M.

PY - 2006

Y1 - 2006

N2 - Background: Gambierol is a polycyclic ether toxin with the same biogenetic origin as ciguatoxins. Gambierol has been associated with neurological symptoms in humans even though its mechanism of action has not been fully characterized. Methods: We studied the effect of gambierol in human neuroblastoma cells by using bis-oxonol to measure membrane potential and FURA-2 to monitor intracellular calcium. Results: We found that this toxin: i) produced a membrane depolarization, ii) potentiated the effect of veratridine on membrane potential iii) decreased ciguatoxin-induced depolarization and iv) increased cytosolic calcium in neuroblastoma cells. Conclusion: These results indicate that gambierol modulate ion fluxes by acting as a partial agonist of sodium channels.

AB - Background: Gambierol is a polycyclic ether toxin with the same biogenetic origin as ciguatoxins. Gambierol has been associated with neurological symptoms in humans even though its mechanism of action has not been fully characterized. Methods: We studied the effect of gambierol in human neuroblastoma cells by using bis-oxonol to measure membrane potential and FURA-2 to monitor intracellular calcium. Results: We found that this toxin: i) produced a membrane depolarization, ii) potentiated the effect of veratridine on membrane potential iii) decreased ciguatoxin-induced depolarization and iv) increased cytosolic calcium in neuroblastoma cells. Conclusion: These results indicate that gambierol modulate ion fluxes by acting as a partial agonist of sodium channels.

KW - Gambierol

KW - Neuroblastoma

KW - Sodium channel

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The sodium channel of human excitable cells is a target for gambierol

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