@article{02e80e2976d34913a09beabc7137ccc4,
title = "The transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program",
abstract = "Mature lymphoid cells express the transcription repressor Bach2, which imposes regulation on humoral and cellular immunity. Here we found critical roles for Bach2 in the development of cells of the B lineage, commencing from the common lymphoid progenitor (CLP) stage, with Bach1 as an auxiliary. Overexpression of Bach2 in pre-pro-B cells deficient in the transcription factor EBF1 and single-cell analysis of CLPs revealed that Bach2 and Bach1 repressed the expression of genes important for myeloid cells ('myeloid genes'). Bach2 and Bach1 bound to presumptive regulatory regions of the myeloid genes. Bach2 hi CLPs showed resistance to myeloid differentiation even when cultured under myeloid conditions. Our results suggest that Bach2 functions with Bach1 and EBF1 to promote B cell development by repressing myeloid genes in CLPs.",
author = "Ari Itoh-Nakadai and Reina Hikota and Akihiko Muto and Kohei Kometani and Miki Watanabe-Matsui and Yuki Sato and Masahiro Kobayashi and Atsushi Nakamura and Yuichi Miura and Yoko Yano and Satoshi Tashiro and Jiying Sun and Tomokatsu Ikawa and Kyoko Ochiai and Tomohiro Kurosaki and Kazuhiko Igarashi",
note = "Funding Information: We thank H. Singh (Cincinnati Children{\textquoteright}s Hospital Medical Center) for discussions, the Ebf1 retroviral construct and Ebf1−/− cells; M.R. Clark (University of Chicago) for the retroviral construct encoding constitutively active STAT5b; K. Takatsu (Toyama University) for OP9 stroma cells; T. Iino, A. Akashi, A. Brydun and M. Matsumoto for advice on DNA microarray analysis; A. Brydun for the generation of antibody to Bach1; R. Yamashita for advice on the analysis of modules from the Immunological Genome Project; H. Kawamoto and members of the Igarashi laboratory for discussions; A. Arakawa for help in the generation of Bach2 reporter mice; K. Watanabe for help with experiments; M. Satake for discussions about repressors; and the Biomedical Research Core of Tohoku University Graduate School of Medicine for their technical support. Supported by Grants-in-Aid from the Japan Society for the Promotion of Science (09J07369 to A.I.-N.; 21229007 to T.K.; 00250738, 21249014 and 00250738 to K.I.), the Network Medicine Global-COE Program of the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Uehara Foundation, the Takeda Foundation, the NOVARTIS Foundation (Japan) for the Promotion of Science and Astellas Foundation for Research on Metabolic Disorders, and the Japan Society for the Promotion of Science and the Tohoku University Institute for International Advanced Research and Education (A.I.-N.).",
year = "2014",
month = nov,
day = "18",
doi = "10.1038/ni.3024",
language = "English",
volume = "15",
pages = "1171--1180",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "12",
}