The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination

Erina Iwabuchi, Yasuhiro Miki, Hironobu Sasano

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)

Abstract

The therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore, it is important to focus on PPIs. This review summarizes the PPIs of ER and HER2 in breast cancer, especially those using a proximity ligation assay that can visualize PPIs in FFPE tissues. In particular, assessing the interaction of CEACAM6 with HER2 may serve as a surrogate marker for the efficacy of trastuzumab in patients with breast cancer. Therefore, in this review, the technique used to detect the interaction of CEACAM6 and HER2 in routinely processed pathological specimens will be applied to the clinical practice of drug selection. We showed the possibility as a novel pathological examination method using PPIs.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalActa Histochemica et Cytochemica
Volume54
Issue number6
DOIs
Publication statusPublished - 2021

Keywords

  • Breast cancer
  • Estrogen receptor
  • Human epidermal growth factor receptor 2
  • Protein-protein interaction
  • Proximity ligation assay

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