TY - JOUR
T1 - Therapeutic potential of multilineage-differentiating stress-enduring cells for osteochondral repair in a rat model
AU - Mahmoud, Elhussein Elbadry
AU - Kamei, Naosuke
AU - Shimizu, Ryo
AU - Wakao, Shohei
AU - Dezawa, Mari
AU - Adachi, Nobuo
AU - Ochi, Mitsuo
N1 - Funding Information:
The authors would like to thank Takako Miyata for her technical support. The authors would like to express their appreciation to the animal facility of Natural Science Center for Basic Research and Development at Hiroshima University for the use of their facilities. This work was supported in part by a grant-in-aid to Professor Mitsuo Ochi for scientific research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (no. 25253089).
Publisher Copyright:
© 2017 Elhussein Elbadry Mahmoud et al.
PY - 2017
Y1 - 2017
N2 - Multilineage-differentiating stress-enduring (Muse) cells are stage-specific embryonic antigen-3 (SSEA-3) positive cells existing in mesenchymal stem cell (MSC) populations. Muse cells have the pluripotency to differentiate into all germ layers as embryonic stem cells. In this study, we aimed to investigate the efficacy of Muse cell transplantation for osteochondral defect repair. Muse cells were isolated from human bone marrow MSCs. An osteochondral defect was created in the patellar groove of immunodeficient rats. After this, cell injection was performed, whereby rats were divided into 3 groups: the control group, the rats of which were given a PBS injection; the non-Muse group, which comprised 5 × 104 SSEA-3 negative non-Muse cells; and the Muse group, which comprised 5 × 104 SSEA-3 positive Muse cells. The white repaired tissue had a mostly smooth homogenous surface at 12 weeks after treatment in the Muse group, while no repair tissue was detected in the control and non-Muse groups. Histological assessments showed better repair at the cartilage defect sites in the Muse group compared to the other groups at 4 and 12 weeks after treatment. Muse cells could be a new promising cell source for the treatment of osteochondral defects.
AB - Multilineage-differentiating stress-enduring (Muse) cells are stage-specific embryonic antigen-3 (SSEA-3) positive cells existing in mesenchymal stem cell (MSC) populations. Muse cells have the pluripotency to differentiate into all germ layers as embryonic stem cells. In this study, we aimed to investigate the efficacy of Muse cell transplantation for osteochondral defect repair. Muse cells were isolated from human bone marrow MSCs. An osteochondral defect was created in the patellar groove of immunodeficient rats. After this, cell injection was performed, whereby rats were divided into 3 groups: the control group, the rats of which were given a PBS injection; the non-Muse group, which comprised 5 × 104 SSEA-3 negative non-Muse cells; and the Muse group, which comprised 5 × 104 SSEA-3 positive Muse cells. The white repaired tissue had a mostly smooth homogenous surface at 12 weeks after treatment in the Muse group, while no repair tissue was detected in the control and non-Muse groups. Histological assessments showed better repair at the cartilage defect sites in the Muse group compared to the other groups at 4 and 12 weeks after treatment. Muse cells could be a new promising cell source for the treatment of osteochondral defects.
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U2 - 10.1155/2017/8154569
DO - 10.1155/2017/8154569
M3 - Article
AN - SCOPUS:85042622299
SN - 1687-966X
VL - 2017
JO - Stem Cells International
JF - Stem Cells International
M1 - 8154569
ER -