TY - JOUR
T1 - Three-dimensional cell culture device utilizing thin membrane deformation by decompression
AU - Anada, Takahisa
AU - Masuda, Taisuke
AU - Honda, Yoshitomo
AU - Fukuda, Junji
AU - Arai, Fumihito
AU - Fukuda, Toshio
AU - Suzuki, Osamu
PY - 2010/5/18
Y1 - 2010/5/18
N2 - We have developed a novel multicellular aggregate (spheroid) culture device that utilizes thin polydimethylsiloxane membrane deformation by decompression. The device was capable of producing thousands of spheroids rapidly at a time and regulating the diameter of spheroids with narrow size distribution. The spheroids were easily retrieved from the device noninvasively. These characteristics of the device suggest that it has the potential for use in a wide variety of applications, such as drug screening and tissue engineering.
AB - We have developed a novel multicellular aggregate (spheroid) culture device that utilizes thin polydimethylsiloxane membrane deformation by decompression. The device was capable of producing thousands of spheroids rapidly at a time and regulating the diameter of spheroids with narrow size distribution. The spheroids were easily retrieved from the device noninvasively. These characteristics of the device suggest that it has the potential for use in a wide variety of applications, such as drug screening and tissue engineering.
KW - Cell culture device
KW - Polydimethylsiloxane
KW - Spheroids
UR - http://www.scopus.com/inward/record.url?scp=77953129285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953129285&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2010.01.065
DO - 10.1016/j.snb.2010.01.065
M3 - Article
AN - SCOPUS:77953129285
SN - 0925-4005
VL - 147
SP - 376
EP - 379
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1
ER -