Three-dimensional cell culture device utilizing thin membrane deformation by decompression

Takahisa Anada, Taisuke Masuda, Yoshitomo Honda, Junji Fukuda, Fumihito Arai, Toshio Fukuda, Osamu Suzuki

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


We have developed a novel multicellular aggregate (spheroid) culture device that utilizes thin polydimethylsiloxane membrane deformation by decompression. The device was capable of producing thousands of spheroids rapidly at a time and regulating the diameter of spheroids with narrow size distribution. The spheroids were easily retrieved from the device noninvasively. These characteristics of the device suggest that it has the potential for use in a wide variety of applications, such as drug screening and tissue engineering.

Original languageEnglish
Pages (from-to)376-379
Number of pages4
JournalSensors and Actuators B: Chemical
Issue number1
Publication statusPublished - 2010 May 18


  • Cell culture device
  • Polydimethylsiloxane
  • Spheroids


Dive into the research topics of 'Three-dimensional cell culture device utilizing thin membrane deformation by decompression'. Together they form a unique fingerprint.

Cite this