Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test

Yu Suke Torisawa; Hitoshi Shiku; Tomoyuki Yasukawa; Matsuhiko Nishizawa; Tomokazu Matsue

doi:10.1016/j.snb.2004.11.045

Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test

Yu Suke Torisawa, Hitoshi Shiku, Tomoyuki Yasukawa, Matsuhiko Nishizawa, Tomokazu Matsue

Tohoku University

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The oxygen consumption rate of three-dimensional cultured cells integrated in a silicon chip was non-invasively quantified by scanning electrochemical microscopy (SECM). The cells were embedded in a collagen gel matrix and entrapped in silicon microstructures. The oxygen concentration profile near the cell panel agreed well with the theoretically determined spherical diffusion. The oxygen consumption rate (F) of a single cell was calculated as (1.0 ± 0.19) × 10^-16 mol s^-1. The oxygen consumption of a cell panel increased along with the cellular proliferation; the increase in respiration activity was in accordance with the increase in cell numbers. Our three-dimensional micro-culture system combined with SECM enables continuous quantification of the oxygen consumption rate using very small amounts of sample.

Original language	English
Pages (from-to)	654-659
Number of pages	6
Journal	Sensors and Actuators B: Chemical
Volume	108
Issue number	1-2 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.snb.2004.11.045
Publication status	Published - 2005 Jul 22

Keywords

Cell chip
Collagen gel
Scanning electrochemical microscopy
Three-dimensional cell culture

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10.1016/j.snb.2004.11.045

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title = "Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test",

abstract = "The oxygen consumption rate of three-dimensional cultured cells integrated in a silicon chip was non-invasively quantified by scanning electrochemical microscopy (SECM). The cells were embedded in a collagen gel matrix and entrapped in silicon microstructures. The oxygen concentration profile near the cell panel agreed well with the theoretically determined spherical diffusion. The oxygen consumption rate (F) of a single cell was calculated as (1.0 ± 0.19) × 10-16 mol s-1. The oxygen consumption of a cell panel increased along with the cellular proliferation; the increase in respiration activity was in accordance with the increase in cell numbers. Our three-dimensional micro-culture system combined with SECM enables continuous quantification of the oxygen consumption rate using very small amounts of sample.",

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note = "Funding Information: This work was partly supported by Grants-in-Aid for Scientific Research (nos. 15033204 and 15201030) from the Ministry of Education, Science and Culture, Japan. ",

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AU - Torisawa, Yu Suke

AU - Shiku, Hitoshi

AU - Yasukawa, Tomoyuki

AU - Nishizawa, Matsuhiko

AU - Matsue, Tomokazu

N1 - Funding Information: This work was partly supported by Grants-in-Aid for Scientific Research (nos. 15033204 and 15201030) from the Ministry of Education, Science and Culture, Japan.

PY - 2005/7/22

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N2 - The oxygen consumption rate of three-dimensional cultured cells integrated in a silicon chip was non-invasively quantified by scanning electrochemical microscopy (SECM). The cells were embedded in a collagen gel matrix and entrapped in silicon microstructures. The oxygen concentration profile near the cell panel agreed well with the theoretically determined spherical diffusion. The oxygen consumption rate (F) of a single cell was calculated as (1.0 ± 0.19) × 10-16 mol s-1. The oxygen consumption of a cell panel increased along with the cellular proliferation; the increase in respiration activity was in accordance with the increase in cell numbers. Our three-dimensional micro-culture system combined with SECM enables continuous quantification of the oxygen consumption rate using very small amounts of sample.

AB - The oxygen consumption rate of three-dimensional cultured cells integrated in a silicon chip was non-invasively quantified by scanning electrochemical microscopy (SECM). The cells were embedded in a collagen gel matrix and entrapped in silicon microstructures. The oxygen concentration profile near the cell panel agreed well with the theoretically determined spherical diffusion. The oxygen consumption rate (F) of a single cell was calculated as (1.0 ± 0.19) × 10-16 mol s-1. The oxygen consumption of a cell panel increased along with the cellular proliferation; the increase in respiration activity was in accordance with the increase in cell numbers. Our three-dimensional micro-culture system combined with SECM enables continuous quantification of the oxygen consumption rate using very small amounts of sample.

KW - Cell chip

KW - Collagen gel

KW - Scanning electrochemical microscopy

KW - Three-dimensional cell culture

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Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test

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Keywords

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