Thrombopoietin has a direct effect on erythroid differentiation

M. Yamada, N. Komatsu, K. Kirito, R. Shimizu, T. Kato, R. Mivazak, P. K. Abe, Y. Miura

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The administration of thrombopoietin (TPO) to mice after chemotherapy and total body irradiation not only promotes recovery from chemotherapy-induced thrombocytopenia, but also relieves anemia. In addition, TPO has burst promoting activity (BPA) in vitro. These observations suggest the influence of TPO on erythropoiesis. To elucidate whether TPO has a direct effect on erythroid differentiation, we established a subclone of a human cytokine-dependent cell line UT7, UT-7/GM Hl. UT-7/GM HI is maintained by GM-CSF and can grow in response to erythropoietin (EPO) or TPO. After a 1 week culture in IMDM medium containing 10% FCS in the presence of I U/ml of EPO or 10 ng/ml of TPO, more than 90 % and 45 % of UT-7/GM HI cells, respectively, were dianisidine-stained. Even after treatment of UT7/GM HI cells with up to 100 ng/ml of TPO, the EPO receptor was not tyrosine phosphorylated. These findings suggest that TPO directly induced erythroid differentiation and that the effect was not achieved via an erythropoietin receptor.

Original languageEnglish
Pages (from-to)1108
Number of pages1
JournalExperimental Hematology
Issue number9
Publication statusPublished - 1996


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