Tissue distribution of tetrodotoxin in the red-spotted newt Notophthalmus viridescens

Dietrich Mebs; Osamu Arakawa; Mari Yotsu-Yamashita

doi:10.1016/j.toxicon.2010.02.009

Tissue distribution of tetrodotoxin in the red-spotted newt Notophthalmus viridescens

Dietrich Mebs, Osamu Arakawa, Mari Yotsu-Yamashita

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

High levels of tetrodotoxin (TTX) and of its analogue 6-epi-tetrodotoxin have been detected in skin and liver extracts of the red-spotted newt Notophthalmus viridescens assayed by a post-column fluorescent-HPLC system. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the granular glands of the epidermis as well as in the muscle layer between the muscle fibres, but also in most organs suggesting that TTX is stored in the whole body of the newt and is secreted by skin glands.

Original language	English
Pages (from-to)	1353-1357
Number of pages	5
Journal	Toxicon
Volume	55
Issue number	7
DOIs	https://doi.org/10.1016/j.toxicon.2010.02.009
Publication status	Published - 2010 Jun

Keywords

6-epi-tetrodotoxin
Monoclonal antibody
Newt
Notophthalmus viridescens
Organ distribution
Tetrodotoxin

ASJC Scopus subject areas

Toxicology

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10.1016/j.toxicon.2010.02.009

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@article{87f083c7c47c463496ef86e29ea35681,

title = "Tissue distribution of tetrodotoxin in the red-spotted newt Notophthalmus viridescens",

abstract = "High levels of tetrodotoxin (TTX) and of its analogue 6-epi-tetrodotoxin have been detected in skin and liver extracts of the red-spotted newt Notophthalmus viridescens assayed by a post-column fluorescent-HPLC system. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the granular glands of the epidermis as well as in the muscle layer between the muscle fibres, but also in most organs suggesting that TTX is stored in the whole body of the newt and is secreted by skin glands.",

keywords = "6-epi-tetrodotoxin, Monoclonal antibody, Newt, Notophthalmus viridescens, Organ distribution, Tetrodotoxin",

author = "Dietrich Mebs and Osamu Arakawa and Mari Yotsu-Yamashita",

note = "Funding Information: We like to express our sincere thanks to Dr. Kentaro Kawatsu, Osaka Prefectural Institute of Public Health for providing the TTX-antibodies. Christine Elbert has performed excellent immunohistological work. This work was partially supported in part by Grants-in-Aid for Scientific Research from Ministry of Education, Science and Culture of Japan JSPS (No. 21380070 to M. Y. Y.). ",

year = "2010",

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doi = "10.1016/j.toxicon.2010.02.009",

language = "English",

volume = "55",

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T1 - Tissue distribution of tetrodotoxin in the red-spotted newt Notophthalmus viridescens

AU - Mebs, Dietrich

AU - Arakawa, Osamu

AU - Yotsu-Yamashita, Mari

N1 - Funding Information: We like to express our sincere thanks to Dr. Kentaro Kawatsu, Osaka Prefectural Institute of Public Health for providing the TTX-antibodies. Christine Elbert has performed excellent immunohistological work. This work was partially supported in part by Grants-in-Aid for Scientific Research from Ministry of Education, Science and Culture of Japan JSPS (No. 21380070 to M. Y. Y.).

PY - 2010/6

Y1 - 2010/6

N2 - High levels of tetrodotoxin (TTX) and of its analogue 6-epi-tetrodotoxin have been detected in skin and liver extracts of the red-spotted newt Notophthalmus viridescens assayed by a post-column fluorescent-HPLC system. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the granular glands of the epidermis as well as in the muscle layer between the muscle fibres, but also in most organs suggesting that TTX is stored in the whole body of the newt and is secreted by skin glands.

AB - High levels of tetrodotoxin (TTX) and of its analogue 6-epi-tetrodotoxin have been detected in skin and liver extracts of the red-spotted newt Notophthalmus viridescens assayed by a post-column fluorescent-HPLC system. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the granular glands of the epidermis as well as in the muscle layer between the muscle fibres, but also in most organs suggesting that TTX is stored in the whole body of the newt and is secreted by skin glands.

KW - 6-epi-tetrodotoxin

KW - Monoclonal antibody

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KW - Notophthalmus viridescens

KW - Organ distribution

KW - Tetrodotoxin

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Tissue distribution of tetrodotoxin in the red-spotted newt Notophthalmus viridescens

Abstract

Keywords

ASJC Scopus subject areas

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