Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals

Junya Uwayama; Aki Hirayama; Toru Yanagawa; Eiji Warabi; Rika Sugimoto; Ken Itoh; Masayuki Yamamoto; Hiroshi Yoshida; Akio Koyama; Tetsuro Ishii

doi:10.1016/j.bbrc.2005.10.192

Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals

Junya Uwayama, Aki Hirayama, Toru Yanagawa, Eiji Warabi, Rika Sugimoto, Ken Itoh, Masayuki Yamamoto, Hiroshi Yoshida, Akio Koyama, Tetsuro Ishii

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I ^-/- mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I^-/- than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I^-/- mice. These results demonstrate that Prx I^-/- mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.

Original language	English
Pages (from-to)	226-231
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	339
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.10.192
Publication status	Published - 2006 Jan 6
Externally published	Yes

Keywords

Carbamoyl-PROXYL
EPR imaging
Ferric-nitrilotriacetate
Free radicals
OmniBank
Peroxiredoxin I

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.10.192

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title = "Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals",

abstract = "Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I -/- mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I-/- than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I-/- mice. These results demonstrate that Prx I-/- mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.",

keywords = "Carbamoyl-PROXYL, EPR imaging, Ferric-nitrilotriacetate, Free radicals, OmniBank, Peroxiredoxin I",

author = "Junya Uwayama and Aki Hirayama and Toru Yanagawa and Eiji Warabi and Rika Sugimoto and Ken Itoh and Masayuki Yamamoto and Hiroshi Yoshida and Akio Koyama and Tetsuro Ishii",

note = "Funding Information: We thank Professor G.E. Mann for his comments on this study. This work was supported by grants from University of Tsukuba Research Projects, and from the Japanese Society for Promotion of Science to T.I. (15310033), T.Y. (16591983), A.H. (15790427), and H.Y. (14370656).",

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doi = "10.1016/j.bbrc.2005.10.192",

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AU - Uwayama, Junya

AU - Hirayama, Aki

AU - Yanagawa, Toru

AU - Warabi, Eiji

AU - Sugimoto, Rika

AU - Itoh, Ken

AU - Yamamoto, Masayuki

AU - Yoshida, Hiroshi

AU - Koyama, Akio

AU - Ishii, Tetsuro

N1 - Funding Information: We thank Professor G.E. Mann for his comments on this study. This work was supported by grants from University of Tsukuba Research Projects, and from the Japanese Society for Promotion of Science to T.I. (15310033), T.Y. (16591983), A.H. (15790427), and H.Y. (14370656).

PY - 2006/1/6

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N2 - Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I -/- mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I-/- than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I-/- mice. These results demonstrate that Prx I-/- mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.

AB - Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I -/- mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I-/- than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I-/- mice. These results demonstrate that Prx I-/- mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.

KW - Carbamoyl-PROXYL

KW - EPR imaging

KW - Ferric-nitrilotriacetate

KW - Free radicals

KW - OmniBank

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Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals

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Keywords

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