Toll-like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes

Saaya Koike; Kenshi Yamasaki; Takeshi Yamauchi; Mai Inoue; Ryoko Shimada-Ohmori; Kenichiro Tsuchiyama; Setsuya Aiba

doi:10.1111/pcmr.12703

Toll-like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes

Saaya Koike, Kenshi Yamasaki, Takeshi Yamauchi, Mai Inoue, Ryoko Shimada-Ohmori, Kenichiro Tsuchiyama, Setsuya Aiba

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100-positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes.

Original language	English
Pages (from-to)	570-584
Number of pages	15
Journal	Pigment Cell and Melanoma Research
Volume	31
Issue number	5
DOIs	https://doi.org/10.1111/pcmr.12703
Publication status	Published - 2018 Sept

Keywords

Rab27A
melanogenesis
melanosome
toll-like receptor
ultraviolet

ASJC Scopus subject areas

Oncology
Biochemistry, Genetics and Molecular Biology(all)
Dermatology

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10.1111/pcmr.12703

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title = "Toll-like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes",

abstract = "Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100-positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes.",

keywords = "Rab27A, melanogenesis, melanosome, toll-like receptor, ultraviolet",

author = "Saaya Koike and Kenshi Yamasaki and Takeshi Yamauchi and Mai Inoue and Ryoko Shimada-Ohmori and Kenichiro Tsuchiyama and Setsuya Aiba",

note = "Funding Information: This work was supported in part by Grant-in-aid for Challenging Exploratory Research 16K15542 (K.Y.) and a Grant-in-aid for Scientific Research C 24591622 (K.Y.) and 25893012 (K.T) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, by Novartis Pharma Research Grants (K.Y.), and by research grant from Kao Melanin Workshop (K.Y.). Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2018",

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doi = "10.1111/pcmr.12703",

language = "English",

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AU - Koike, Saaya

AU - Yamasaki, Kenshi

AU - Yamauchi, Takeshi

AU - Inoue, Mai

AU - Shimada-Ohmori, Ryoko

AU - Tsuchiyama, Kenichiro

AU - Aiba, Setsuya

N1 - Funding Information: This work was supported in part by Grant-in-aid for Challenging Exploratory Research 16K15542 (K.Y.) and a Grant-in-aid for Scientific Research C 24591622 (K.Y.) and 25893012 (K.T) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, by Novartis Pharma Research Grants (K.Y.), and by research grant from Kao Melanin Workshop (K.Y.). Publisher Copyright: © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

PY - 2018/9

Y1 - 2018/9

N2 - Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100-positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes.

AB - Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100-positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes.

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Toll-like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes

Abstract

Keywords

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