TY - JOUR
T1 - Tos17 insertion in NADH-dependent glutamate synthase genes leads to an increase in grain protein content in rice
AU - Imagawa, Fumi
AU - Minagawa, Haruka
AU - Nakayama, Yosuke
AU - Kanno, Keiichi
AU - Hayakawa, Toshihiko
AU - Kojima, Soichi
N1 - Funding Information:
SK was supported by Tojuro Iijima Foundation for Food Science and Technology ( H27_19 ), and TH was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (KIBAN B; 17H03780 ).
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/11
Y1 - 2018/11
N2 - Nutrition from cereal grains sustains human life, and the protein content of rice grains is an important source for humans. Recent forward genetics studies pointed out the significant contribution of NADH-dependent glutamate synthase (NADH-GOGAT) to increased grain protein content in durum wheat. The aim of this work was to investigate the contribution of the two NADH-GOGAT enzymes in rice, NADH-GOGAT1, and NADH-GOGAT2, to rice grain protein content, using a reverse genetics approach. The grain protein content and free amino acid concentration were determined in Tos17 insertion mutants of the structural gene of each protein. We found a significant increase in protein content in the NADH-GOGAT2 mutant and an increase in free amino acid concentration in both the NADH-GOGAT1 and the NADH-GOGAT2 mutants. These results provide the first nutritional characterization of the NADH-GOGAT mutants. Rice containing increased protein and amino acid concentrations may have potential to contribute to improved rice nutrition. Since previous field analysis had indicated a 40% yield reduction in each of the mutants, future work should focus on improving the yield of the mutants.
AB - Nutrition from cereal grains sustains human life, and the protein content of rice grains is an important source for humans. Recent forward genetics studies pointed out the significant contribution of NADH-dependent glutamate synthase (NADH-GOGAT) to increased grain protein content in durum wheat. The aim of this work was to investigate the contribution of the two NADH-GOGAT enzymes in rice, NADH-GOGAT1, and NADH-GOGAT2, to rice grain protein content, using a reverse genetics approach. The grain protein content and free amino acid concentration were determined in Tos17 insertion mutants of the structural gene of each protein. We found a significant increase in protein content in the NADH-GOGAT2 mutant and an increase in free amino acid concentration in both the NADH-GOGAT1 and the NADH-GOGAT2 mutants. These results provide the first nutritional characterization of the NADH-GOGAT mutants. Rice containing increased protein and amino acid concentrations may have potential to contribute to improved rice nutrition. Since previous field analysis had indicated a 40% yield reduction in each of the mutants, future work should focus on improving the yield of the mutants.
KW - GABA
KW - Rice
KW - Seed storage protein
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U2 - 10.1016/j.jcs.2018.09.008
DO - 10.1016/j.jcs.2018.09.008
M3 - Article
AN - SCOPUS:85054310554
SN - 0733-5210
VL - 84
SP - 38
EP - 43
JO - Journal of Cereal Science
JF - Journal of Cereal Science
ER -