Trading polymeric microspheres: Exchanging DNA molecules via microsphere interaction

Nobuyuki Morimoto, Kanna Muramatsu, Shin ichiro M. Nomura, Makoto Suzuki

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


A new class of artificial molecular transport system is constructed by polymeric microspheres. The microspheres are prepared by self-assembly of poly(ethylene glycol)-block-poly(3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate), PEG-b-PDMAPS, by intermolecular dipole-dipole interaction of sulfobetaine side chains in water. Below the upper critical solution temperature (UCST) of PEG-b-PDMAPS, the microspheres (~1. μm) interact with other microspheres by partial and transit fusion. In order to apply the interaction between microspheres, a 3'-TAMRA-labeled single-stranded DNA oligomer (ssDNA) is encapsulated into a PEG-b-PDMAPS microsphere by thermal treatment. The exchange of ssDNA between microspheres is confirmed by fluorescence resonance energy transfer (FRET) quenching derived from double-stranded formation with complementary 5'-BHQ-2-labeled ssDNA encapsulated in PEG-b-PDMAPS microspheres. The exchange rate of ssDNA is controllable by tuning the composition of the polymer. The contact-dependent transport of molecules can be applied in the areas of microreactors, sensor devices, etc.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 2015 Apr 1


  • Double strand formation
  • Microspheres
  • Self-assembly
  • Single-stranded DNA oligomer
  • Zwitterionic block copolymers


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