TY - JOUR
T1 - Trading polymeric microspheres
T2 - Exchanging DNA molecules via microsphere interaction
AU - Morimoto, Nobuyuki
AU - Muramatsu, Kanna
AU - Nomura, Shin ichiro M.
AU - Suzuki, Makoto
N1 - Funding Information:
The authors wish to thank Dr. Yuichi Inoue (Tohoku University) for assisting with microscopic observations. N.M. acknowledges the funding support for this work from Grants-in-Aid for Scientific Research ( #25350549 ) from the Japan Society for the Promotion of Science (JSPS) and Program Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - A new class of artificial molecular transport system is constructed by polymeric microspheres. The microspheres are prepared by self-assembly of poly(ethylene glycol)-block-poly(3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate), PEG-b-PDMAPS, by intermolecular dipole-dipole interaction of sulfobetaine side chains in water. Below the upper critical solution temperature (UCST) of PEG-b-PDMAPS, the microspheres (~1. μm) interact with other microspheres by partial and transit fusion. In order to apply the interaction between microspheres, a 3'-TAMRA-labeled single-stranded DNA oligomer (ssDNA) is encapsulated into a PEG-b-PDMAPS microsphere by thermal treatment. The exchange of ssDNA between microspheres is confirmed by fluorescence resonance energy transfer (FRET) quenching derived from double-stranded formation with complementary 5'-BHQ-2-labeled ssDNA encapsulated in PEG-b-PDMAPS microspheres. The exchange rate of ssDNA is controllable by tuning the composition of the polymer. The contact-dependent transport of molecules can be applied in the areas of microreactors, sensor devices, etc.
AB - A new class of artificial molecular transport system is constructed by polymeric microspheres. The microspheres are prepared by self-assembly of poly(ethylene glycol)-block-poly(3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate), PEG-b-PDMAPS, by intermolecular dipole-dipole interaction of sulfobetaine side chains in water. Below the upper critical solution temperature (UCST) of PEG-b-PDMAPS, the microspheres (~1. μm) interact with other microspheres by partial and transit fusion. In order to apply the interaction between microspheres, a 3'-TAMRA-labeled single-stranded DNA oligomer (ssDNA) is encapsulated into a PEG-b-PDMAPS microsphere by thermal treatment. The exchange of ssDNA between microspheres is confirmed by fluorescence resonance energy transfer (FRET) quenching derived from double-stranded formation with complementary 5'-BHQ-2-labeled ssDNA encapsulated in PEG-b-PDMAPS microspheres. The exchange rate of ssDNA is controllable by tuning the composition of the polymer. The contact-dependent transport of molecules can be applied in the areas of microreactors, sensor devices, etc.
KW - Double strand formation
KW - Microspheres
KW - Self-assembly
KW - Single-stranded DNA oligomer
KW - Zwitterionic block copolymers
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U2 - 10.1016/j.colsurfb.2015.02.014
DO - 10.1016/j.colsurfb.2015.02.014
M3 - Article
C2 - 25731098
AN - SCOPUS:84923381504
SN - 0927-7765
VL - 128
SP - 94
EP - 99
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -