TY - JOUR
T1 - Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
AU - Sugimura, Satoshi
AU - Kobayashi, Norio
AU - Okae, Hiroaki
AU - Yamanouchi, Tadayuki
AU - Matsuda, Hideo
AU - Kojima, Takumi
AU - Yajima, Akira
AU - Hashiyada, Yutaka
AU - Kaneda, Masahiro
AU - Sato, Kan
AU - Imai, Kei
AU - Tanemura, Kentaro
AU - Arima, Takahiro
AU - Gilchrist, Robert B.
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number JP15K18768 to S. S. and Kieikai Research Foundation to S. S. Authors thank the members of National Livestock Breeding Center for the management of cows and the kind donation of bull sperm.
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - During antral folliculogenesis, developmental competence of prospective oocytes is regulated in large part by the follicular somatic component to prepare the oocyte for the final stage of maturation and subsequent embryo development. The underlying molecular mechanisms are poorly understood. Oocytes reaching the advanced stage of follicular growth by administration of exogenous follicle-stimulating hormone (FSH) possess higher developmental competence than oocytes in FSH-untreated smaller follicles. In this study, the transcriptomic profile of the cumulus cells from cows receiving FSH administration (FSH-priming) was compared, as a model of high oocyte competence, with that from untreated donor cows (control). Ingenuity Pathway Analysis showed that cumulus cells receiving FSH-priming were rich in down-regulated transcripts associated with cell movement and migration, including the extracellular matrix-related transcripts, probably preventing the disruption of cell-to-cell contacts. Interestingly, the transcriptomic profile of up-regulated genes in the control group was similar to that of granulosa cells from atretic follicles. Interferon regulatory factor 7 was activated as the key upstream regulator of FSH-priming. Thus, acquisition of developmental competence by oocytes can be ensured by the integrity of cumulus cells involved in cell-to-cell communication and cell survival, which may help achieve enhanced oocyte-somatic cell coupling.
AB - During antral folliculogenesis, developmental competence of prospective oocytes is regulated in large part by the follicular somatic component to prepare the oocyte for the final stage of maturation and subsequent embryo development. The underlying molecular mechanisms are poorly understood. Oocytes reaching the advanced stage of follicular growth by administration of exogenous follicle-stimulating hormone (FSH) possess higher developmental competence than oocytes in FSH-untreated smaller follicles. In this study, the transcriptomic profile of the cumulus cells from cows receiving FSH administration (FSH-priming) was compared, as a model of high oocyte competence, with that from untreated donor cows (control). Ingenuity Pathway Analysis showed that cumulus cells receiving FSH-priming were rich in down-regulated transcripts associated with cell movement and migration, including the extracellular matrix-related transcripts, probably preventing the disruption of cell-to-cell contacts. Interestingly, the transcriptomic profile of up-regulated genes in the control group was similar to that of granulosa cells from atretic follicles. Interferon regulatory factor 7 was activated as the key upstream regulator of FSH-priming. Thus, acquisition of developmental competence by oocytes can be ensured by the integrity of cumulus cells involved in cell-to-cell communication and cell survival, which may help achieve enhanced oocyte-somatic cell coupling.
UR - http://www.scopus.com/inward/record.url?scp=85026375195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026375195&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-07039-5
DO - 10.1038/s41598-017-07039-5
M3 - Article
C2 - 28755009
AN - SCOPUS:85026375195
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 6815
ER -