Transcripts immunoprecipitated with Sxl protein in primordial germ cells of Drosophila embryos

Ryoma Ota, Shumpei Morita, Masanao Sato, Shuji Shigenobu, Makoto Hayashi, Satoru Kobayashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


In Drosophila, Sex lethal (Sxl), an RNA binding protein, is required for induction of female sexual identity in both somatic and germline cells. Although the Sxl-dependent feminizing pathway in the soma was previously elucidated, the downstream targets for Sxl in the germline remained elusive. To identify these target genes, we selected transcripts associated with Sxl in primordial germ cells (PGCs) of embryos using RNA immunoprecipitation coupled to sequencing (RIP-seq) analysis. A total of 308 transcripts encoded by 282 genes were obtained. Seven of these genes, expressed at higher levels in PGCs as determined by microarray and in situ hybridization analyses, were subjected to RNAi-mediated functional analyses. Knockdown of Neos, Kap-alpha3, and CG32075 throughout germline development caused gonadal dysgenesis in a sex-dependent manner, and Su(var)2-10 knockdown caused gonadal dysgenesis in both sexes. Moreover, as with knockdown of Sxl, knockdown of Su(var)2-10 in PGCs gave rise to a tumorous phenotype of germline cells in ovaries. Because this phenotype indicates loss of female identity of germline cells, we consider Su(var)2-10 to be a strong candidate target of Sxl in PGCs. Our results represent a first step toward elucidating the Sxl-dependent feminizing pathway in the germline.

Original languageEnglish
Pages (from-to)713-723
Number of pages11
JournalDevelopment Growth and Differentiation
Issue number9
Publication statusPublished - 2017 Dec
Externally publishedYes


  • germline
  • RNA immunoprecipitation and RNA sequencing
  • sex determination
  • Su(var)2-10
  • Sxl

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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