Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC

Osamu Yamada; Byung Rho Lee; Katsuya Gomi

doi:10.1271/bbb.61.1367

Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC

Osamu Yamada, Byung Rho Lee, Katsuya Gomi

Research output: Contribution to journal › Article › peer-review

117 Citations (Scopus)

Abstract

We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC⁻ mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD⁻ mutant strain of A. oryzae was bestowed with the sC⁻ mutation. Transformation of the A. oryzae host (niaD⁻, sC⁻) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.

Original language	English
Pages (from-to)	1367-1369
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	8
DOIs	https://doi.org/10.1271/bbb.61.1367
Publication status	Published - 1997 Jan
Externally published	Yes

Keywords

ATP sulfurylase
Aspergillus oryzae
Nitrate reductase
Transformation

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC",

abstract = "We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC− mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD− mutant strain of A. oryzae was bestowed with the sC− mutation. Transformation of the A. oryzae host (niaD−, sC−) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.",

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author = "Osamu Yamada and {Rho Lee}, Byung and Katsuya Gomi",

year = "1997",

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language = "English",

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AU - Yamada, Osamu

AU - Rho Lee, Byung

AU - Gomi, Katsuya

PY - 1997/1

Y1 - 1997/1

N2 - We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC− mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD− mutant strain of A. oryzae was bestowed with the sC− mutation. Transformation of the A. oryzae host (niaD−, sC−) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.

AB - We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC− mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD− mutant strain of A. oryzae was bestowed with the sC− mutation. Transformation of the A. oryzae host (niaD−, sC−) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.

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KW - Aspergillus oryzae

KW - Nitrate reductase

KW - Transformation

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Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC

Abstract

Keywords

ASJC Scopus subject areas

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