Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos

Satoshi Namai, Asako Sugimoto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Pristionchus pacificus is a free-living nematode used as a model organism for evolutionary developmental and ecological biology. Although a transgenic technique to form complex arrays by microinjection has been established in P. pacificus, transgene expression from the array in the germline and early embryos tends to be silenced. Here, we established a method to integrate transgenes into the genome of P. pacificus using microparticle bombardment with hygromycin B selection. Additionally, we isolated a mutant exhibiting significantly lower autofluorescence in the germline and early embryos, facilitating visualization of transgene-derived fluorescent proteins for live imaging. Transgenic lines constructed using these tools successfully expressed GFP-tagged proteins in the germline and early embryos and enabled live imaging of chromosomes, microtubules, and centrosomes.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalDevelopment Genes and Evolution
Volume228
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • Microparticle bombardment
  • Pristionchus pacificus
  • Transgenesis

Fingerprint

Dive into the research topics of 'Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos'. Together they form a unique fingerprint.

Cite this