Transgenic Mice with Antisense RNA against the Nucleocapsid Protein Mrna of Mouse Hepatitis Virus

Nobuya Sasaki; Masanobu Hayashi; Shiro Aoyama; Tadashi Yamashita; Shigeo Namioka; Ichiro Miyoshi; Noriyuki Kasai

doi:10.1292/jvms.55.549

Transgenic Mice with Antisense RNA against the Nucleocapsid Protein Mrna of Mouse Hepatitis Virus

Nobuya Sasaki, Masanobu Hayashi, Shiro Aoyama, Tadashi Yamashita, Shigeo Namioka, Ichiro Miyoshi, Noriyuki Kasai

MED - Institute for Animal Experimentation

Hokkaido University

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In this study transgenic mice which expressed antisense RNA against the nucleocapsid protein gene of mouse hepatitis virus (MHV) under the control of RSV LTR were produced. These transgenic mice were able to transmit the foreign gene to their progeny in a Mendelian fashion. Antisense RNA was detected in various tissues from the transgenic mice including liver and brain, the target organs of MHV infection. One strain of transgenic mice derived from founder mouse No.19 was more resistant to the lethal challenge of MHV than non-transgenic mice. The results of the present study show the ability of antisense RNA against the viral gene to protect against viral infection in vivo.

Original language	English
Pages (from-to)	549-554
Number of pages	6
Journal	Journal of Veterinary Medical Science
Volume	55
Issue number	4
DOIs	https://doi.org/10.1292/jvms.55.549
Publication status	Published - 1993

Keywords

antisense RNA
MHV
N protein
transgenic mouse

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1292/jvms.55.549

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abstract = "In this study transgenic mice which expressed antisense RNA against the nucleocapsid protein gene of mouse hepatitis virus (MHV) under the control of RSV LTR were produced. These transgenic mice were able to transmit the foreign gene to their progeny in a Mendelian fashion. Antisense RNA was detected in various tissues from the transgenic mice including liver and brain, the target organs of MHV infection. One strain of transgenic mice derived from founder mouse No.19 was more resistant to the lethal challenge of MHV than non-transgenic mice. The results of the present study show the ability of antisense RNA against the viral gene to protect against viral infection in vivo.",

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AU - Sasaki, Nobuya

AU - Hayashi, Masanobu

AU - Aoyama, Shiro

AU - Yamashita, Tadashi

AU - Namioka, Shigeo

AU - Miyoshi, Ichiro

AU - Kasai, Noriyuki

PY - 1993

Y1 - 1993

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AB - In this study transgenic mice which expressed antisense RNA against the nucleocapsid protein gene of mouse hepatitis virus (MHV) under the control of RSV LTR were produced. These transgenic mice were able to transmit the foreign gene to their progeny in a Mendelian fashion. Antisense RNA was detected in various tissues from the transgenic mice including liver and brain, the target organs of MHV infection. One strain of transgenic mice derived from founder mouse No.19 was more resistant to the lethal challenge of MHV than non-transgenic mice. The results of the present study show the ability of antisense RNA against the viral gene to protect against viral infection in vivo.

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KW - N protein

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Transgenic Mice with Antisense RNA against the Nucleocapsid Protein Mrna of Mouse Hepatitis Virus

Abstract

Keywords

UN SDGs

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