TY - JOUR
T1 - Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3
AU - Yamaguchi, Hiroaki
AU - Okada, Masahiro
AU - Akitaya, Shou
AU - Ohara, Hiroshi
AU - Mikkaichi, Tsuyoshi
AU - Ishikawa, Haruna
AU - Sato, Mayumi
AU - Matsuura, Masaki
AU - Saga, Toshihide
AU - Unno, Michiaki
AU - Abe, Takaaki
AU - Mano, Nariyasu
AU - Hishinuma, Takanori
AU - Goto, Junichi
PY - 2006/6
Y1 - 2006/6
N2 - This study sought to clarify the contributions of organic anion-transporting polypeptide (OATP) 1B1 and 1B3 to the liver uptake of chenodeoxycholic acid (CDCA). We synthesized a fluorescent version of CDCA, chenodeoxychilyl-(Nε-NBD)-lysine (CDCA-NBD), to characterize transporter-mediated uptake. CDCA-NBD is efficiently transported by OATP1B1 and OATP1B3 with high affinities. The Michaelis-Menten constants for CDCA-NBD uptake by OATP1B1 and OATP1B3 were 1.45 ± 0.39 μM and 0.54 ± 0.09 μM, respectively. By confocal laser scanning microscopy, CDCA-NBD, which is taken up by OATP1B1 and OATP1B3, was observed to localize to the cytosol. We also examined the transport of newly synthesized fluorescent bile acids. NBD-labeled bile acids, including cholic acid, deoxycholic acid, lithocholic acid, and ursodeoxycholic acid, were all transported by OATP1B1 and OATP1B3. CDCA-NBD exhibited the highest rate of transport of the five NBD-labeled bile acids examined in OATP1B1- and OATP1B3-expressing cells. Our results suggest that OATP1B1 and OATP1B3 play important roles in CDCA uptake into the liver. Fluorescent bile acids are useful tools to characterize the uptake properties of membrane transporters.
AB - This study sought to clarify the contributions of organic anion-transporting polypeptide (OATP) 1B1 and 1B3 to the liver uptake of chenodeoxycholic acid (CDCA). We synthesized a fluorescent version of CDCA, chenodeoxychilyl-(Nε-NBD)-lysine (CDCA-NBD), to characterize transporter-mediated uptake. CDCA-NBD is efficiently transported by OATP1B1 and OATP1B3 with high affinities. The Michaelis-Menten constants for CDCA-NBD uptake by OATP1B1 and OATP1B3 were 1.45 ± 0.39 μM and 0.54 ± 0.09 μM, respectively. By confocal laser scanning microscopy, CDCA-NBD, which is taken up by OATP1B1 and OATP1B3, was observed to localize to the cytosol. We also examined the transport of newly synthesized fluorescent bile acids. NBD-labeled bile acids, including cholic acid, deoxycholic acid, lithocholic acid, and ursodeoxycholic acid, were all transported by OATP1B1 and OATP1B3. CDCA-NBD exhibited the highest rate of transport of the five NBD-labeled bile acids examined in OATP1B1- and OATP1B3-expressing cells. Our results suggest that OATP1B1 and OATP1B3 play important roles in CDCA uptake into the liver. Fluorescent bile acids are useful tools to characterize the uptake properties of membrane transporters.
KW - Nitrobenz-2-oxa-1,3-diazole
KW - Organic anion-transporting polypeptide 1B1
KW - Organic anion-transporting polypeptide 1B3
KW - Visualization
UR - http://www.scopus.com/inward/record.url?scp=33744785961&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744785961&partnerID=8YFLogxK
U2 - 10.1194/jlr.M500532-JLR200
DO - 10.1194/jlr.M500532-JLR200
M3 - Article
C2 - 16534140
AN - SCOPUS:33744785961
SN - 0022-2275
VL - 47
SP - 1196
EP - 1202
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 6
ER -