Epitaxially grown tetragonal and cubic Mn-Co-Ga thin films were fabricated onto single crystalline Cr (001) under a layer. High perpendicular magnetic anisotropy is achieved in the tetragonal Mn2.3Co0.4Ga 1.3 film, and a small, unexpected perpendicular magnetic anisotropy was induced in the cubic Mn1.8Co1.2Ga1.0 film as well. The tunnel magnetoresistance (TMR) effect of the Mn-Co-Ga/MgO/CoFeB magnetic tunnel junctions (MTJs) were investigated. TMR ratios of 5% and 11% were observed at room temperature for the MTJs using tetragonal Mn 2.3Co0.4Ga1.3 and cubic Mn1.8Co 1.2Ga1.0 electrodes, respectively. The composition dependence is discussed briefly.