Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

Kalyan K. Sadhu; Shin Mizukami; Shuji Watanabe; Kazuya Kikuchi

doi:10.1039/c0cc02432e

Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

Kalyan K. Sadhu, Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.

Original language	English
Pages (from-to)	7403-7405
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	39
DOIs	https://doi.org/10.1039/c0cc02432e
Publication status	Published - 2010 Oct 21
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c0cc02432e

Cite this

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abstract = "The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.",

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AU - Sadhu, Kalyan K.

AU - Mizukami, Shin

AU - Watanabe, Shuji

AU - Kikuchi, Kazuya

PY - 2010/10/21

Y1 - 2010/10/21

N2 - The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.

AB - The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.

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JO - Chemical Communications

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Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

Abstract

ASJC Scopus subject areas

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