Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

Mie Kamoto; Naoki Umezawa; Nobuki Kato; Tsunehiko Higuchi

doi:10.1016/j.bmcl.2009.02.084

Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

Mie Kamoto, Naoki Umezawa, Nobuki Kato, Tsunehiko Higuchi

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.

Original language	English
Pages (from-to)	2285-2288
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	8
DOIs	https://doi.org/10.1016/j.bmcl.2009.02.084
Publication status	Published - 2009 Apr 15
Externally published	Yes

Keywords

Fluorescent probe
Hexahistidine tag
Molecular recognition

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2009.02.084

Cite this

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title = "Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein",

abstract = "We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.",

keywords = "Fluorescent probe, Hexahistidine tag, Molecular recognition",

author = "Mie Kamoto and Naoki Umezawa and Nobuki Kato and Tsunehiko Higuchi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for University and Society Collaboration (12793009) to T.H., and a Grant-in-Aid for Young Scientists (B) (19790094) to N.U. from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and by a Grant-in-Aid for Research in Nagoya City University to N.U. We thank Professor H. Sajiki and Dr. Y. Monguchi (Gifu Pharmaceutical University) for HRMS-FAB analysis. ",

year = "2009",

month = apr,

day = "15",

doi = "10.1016/j.bmcl.2009.02.084",

language = "English",

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journal = "Bioorganic and Medicinal Chemistry Letters",

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T1 - Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

AU - Kamoto, Mie

AU - Umezawa, Naoki

AU - Kato, Nobuki

AU - Higuchi, Tsunehiko

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for University and Society Collaboration (12793009) to T.H., and a Grant-in-Aid for Young Scientists (B) (19790094) to N.U. from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and by a Grant-in-Aid for Research in Nagoya City University to N.U. We thank Professor H. Sajiki and Dr. Y. Monguchi (Gifu Pharmaceutical University) for HRMS-FAB analysis.

PY - 2009/4/15

Y1 - 2009/4/15

N2 - We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.

AB - We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.

KW - Fluorescent probe

KW - Hexahistidine tag

KW - Molecular recognition

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 8

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Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

Abstract

Keywords

ASJC Scopus subject areas

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