Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

Hiroyuki Yokoyama; Hiroshi Tsubokawa; Hengchang Guo; Jun Ichi Shikata; Ki Ichi Sato; Keijiro Takashima; Kaori Kashiwagi; Naoaki Saito; Hirokazu Taniguchi; Hiromasa Ito

doi:10.1117/1.2800393

Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

Hiroyuki Yokoyama, Hiroshi Tsubokawa, Hengchang Guo, Jun Ichi Shikata, Ki Ichi Sato, Keijiro Takashima, Kaori Kashiwagi, Naoaki Saito, Hirokazu Taniguchi, Hiromasa Ito

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

Original language	English
Article number	054019
Journal	Journal of Biomedical Optics
Volume	12
Issue number	5
DOIs	https://doi.org/10.1117/1.2800393
Publication status	Published - 2007 Sept

Keywords

Frequency conversion
Multiphoton processes
Picosecond phenomena
Semiconductor lasers
Ultrafast nonlinear optics fluorescence microscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Biomedical Engineering

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title = "Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source",

abstract = "We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).",

keywords = "Frequency conversion, Multiphoton processes, Picosecond phenomena, Semiconductor lasers, Ultrafast nonlinear optics fluorescence microscopy",

author = "Hiroyuki Yokoyama and Hiroshi Tsubokawa and Hengchang Guo and Shikata, {Jun Ichi} and Sato, {Ki Ichi} and Keijiro Takashima and Kaori Kashiwagi and Naoaki Saito and Hirokazu Taniguchi and Hiromasa Ito",

note = "Funding Information: We are grateful to Central Glass and Sumitomo Osaka Cement for their support in developing the low-nonlinear-effect optical fiber amplifiers. This work was partly supported by the Japan Science and Technology Agency (JST), a Grant-in-Aid for Scientific Research (B) (No. 16360037) from the Japan Society for the Promotion of Science (JSPS), and a research grant from the Collaborative Research Program of the Center for Interdisciplinary Research at Tohoku University. H. C. Guo is supported by a JSPS Postdoctoral Fellowship.",

year = "2007",

month = sep,

doi = "10.1117/1.2800393",

language = "English",

volume = "12",

journal = "Journal of Biomedical Optics",

issn = "1083-3668",

publisher = "SPIE",

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T1 - Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

AU - Yokoyama, Hiroyuki

AU - Tsubokawa, Hiroshi

AU - Guo, Hengchang

AU - Shikata, Jun Ichi

AU - Sato, Ki Ichi

AU - Takashima, Keijiro

AU - Kashiwagi, Kaori

AU - Saito, Naoaki

AU - Taniguchi, Hirokazu

AU - Ito, Hiromasa

N1 - Funding Information: We are grateful to Central Glass and Sumitomo Osaka Cement for their support in developing the low-nonlinear-effect optical fiber amplifiers. This work was partly supported by the Japan Science and Technology Agency (JST), a Grant-in-Aid for Scientific Research (B) (No. 16360037) from the Japan Society for the Promotion of Science (JSPS), and a research grant from the Collaborative Research Program of the Center for Interdisciplinary Research at Tohoku University. H. C. Guo is supported by a JSPS Postdoctoral Fellowship.

PY - 2007/9

Y1 - 2007/9

N2 - We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

AB - We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

KW - Frequency conversion

KW - Multiphoton processes

KW - Picosecond phenomena

KW - Semiconductor lasers

KW - Ultrafast nonlinear optics fluorescence microscopy

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ER -

Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

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Keywords

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