Type I collagen can function as a reservoir of basic fibroblast growth factor

Akihiro Kanematsu, Akira Marui, Shingo Yamamoto, Makoto Ozeki, Yoshiaki Hirano, Masaya Yamamoto, Osamu Ogawa, Masashi Komeda, Yasuhiko Tabata

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)


Storage and release of endogenous growth factors by the extracellular matrix (ECM) are important biological events that control tissue homeostasis and regeneration. The interaction between basic fibroblast growth factor (bFGF) and heparan sulfate proteoglycans has been extensively studied and used as a prototype model of such a system, while the lower affinity of fibrillar type I collagen for bFGF has generally been considered biologically insignificant. However, our present investigation revealed that bFGF spontaneously interacts with type I collagen solution and sponges under in vitro and in vivo physiological conditions, and is protected from the proteolytic environment by the collagen. bFGF incorporated in a collagen sponge sheet was sustainedly released in the mouse subcutis according to the biodegradation of the sponge matrix, and exhibited local angiogenic activity in a dose-dependent manner. Intramuscular injection of collagen microsponges incorporating bFGF induced a significant increase in the blood flow in the murine ischemic hindlimb, which could never have been attained by bolus injection of bFGF. These results suggest the significance and therapeutic utility of type I collagen as a reservoir of bFGF.

Original languageEnglish
Pages (from-to)281-292
Number of pages12
JournalJournal of Controlled Release
Issue number2
Publication statusPublished - 2004 Sept 30


  • Basic fibroblast growth factor
  • Reservoir
  • Type I collagen


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