TY - JOUR
T1 - Unidentified compound specifically accumulated in soybean nodules formed with h2-uptake negative rhizobium japonicum strains
AU - Arima, Yasuhiro
AU - Kumazawa, Kikuo
AU - Minamisawa, Kiwamu
N1 - Funding Information:
Acknowledgments. The authors are grateful to Dr. K. Mikoshiba (Nagano-ken Chushin Agricultural Experiment Station) for supplying soybean seeds, to Dr. M. Kadowaki (The University of Tokyo) for amino acid analysis by HITACHI 835 Amino Acid Analyzer, and to Dr. T. Asami and Dr. M. Kubota (Ibaraki University) for their useful suggestions. This research was supported by a grant of the Ministry of Agriculture, Forestry and Fisheries of Japan (GEP 83-11-10).
PY - 1984/9
Y1 - 1984/9
N2 - A large quantity of an unidentified compound was detected by a fluorogenic method in the soybean nodules formed with H2-uptake negative Rhizobium japonicum strains. The unidentified compound (compound X) reacted with o-phthalaldehyde in the presence of 2-mercaptoethanol giving rise to a fluorescent compound, and was not hydrolyzed in strong acid and strong base. Therefore, the compound X was considered to be one of amines and amino acids. The content of the compound X in the nodules formed with H2-uptake positive strains was remarkably lower than that in the nodules formed with H2-uptake negative strains. It was suggested that the compound X may be involved in the process of hydrogen metabolism in soybean nodules. The compound X was not detected in leaves, stems, petioles, and roots of soybean plants, but detected in H2-uptake negative strains of free-living R. japonicum. In nodules, a great portion of the compound X was distributed into cytosol fraction.
AB - A large quantity of an unidentified compound was detected by a fluorogenic method in the soybean nodules formed with H2-uptake negative Rhizobium japonicum strains. The unidentified compound (compound X) reacted with o-phthalaldehyde in the presence of 2-mercaptoethanol giving rise to a fluorescent compound, and was not hydrolyzed in strong acid and strong base. Therefore, the compound X was considered to be one of amines and amino acids. The content of the compound X in the nodules formed with H2-uptake positive strains was remarkably lower than that in the nodules formed with H2-uptake negative strains. It was suggested that the compound X may be involved in the process of hydrogen metabolism in soybean nodules. The compound X was not detected in leaves, stems, petioles, and roots of soybean plants, but detected in H2-uptake negative strains of free-living R. japonicum. In nodules, a great portion of the compound X was distributed into cytosol fraction.
KW - Amines
KW - Amino acid analysis
KW - Fluorogenic detection
KW - Hydrogenase
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U2 - 10.1080/00380768.1984.10434708
DO - 10.1080/00380768.1984.10434708
M3 - Article
AN - SCOPUS:12644316131
SN - 0038-0768
VL - 30
SP - 435
EP - 444
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
IS - 3
ER -