Up-regulation of brain-derived neurotrophic factor in the dorsal root ganglion of the rat bone cancer pain model

Naoto Tomotsuka, Ryuji Kaku, Norihiko Obata, Yoshikazu Matsuoka, Hirotaka Kanzaki, Arata Taniguchi, Noriko Muto, Hiroki Omiya, Yoshitaro Itano, Tadasu Sato, Hiroyuki Ichikawa, Satoshi Mizobuchi, Hiroshi Morimatsu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Metastatic bone cancer causes severe pain, but current treatments often provide insufficient pain relief. One of the reasons is that mechanisms underlying bone cancer pain are not solved completely. Our previous studies have shown that brain-derived neurotrophic factor (BDNF), known as a member of the neurotrophic family, is an important molecule in the pathological pain state in some pain models. We hypothesized that expression changes of BDNF may be one of the factors related to bone cancer pain; in this study, we investigated changes of BDNF expression in dorsal root ganglia in a rat bone cancer pain model. As we expected, BDNF mRNA (messenger ribonucleic acid) and protein were significantly increased in L3 dorsal root ganglia after intra-tibial inoculation of MRMT-1 rat breast cancer cells. Among the eleven splice-variants of BDNF mRNA, exon 1-9 variant increased predominantly. Interestingly, the up-regulation of BDNF is localized in small neurons (mostly nociceptive neurons) but not in medium or large neurons (non-nociceptive neurons). Further, expression of nerve growth factor (NGF), which is known as a specific promoter of BDNF exon 1-9 variant, was significantly increased in tibial bone marrow. Our findings suggest that BDNF is a key molecule in bone cancer pain, and NGF-BDNF cascade possibly develops bone cancer pain.

Original languageEnglish
Pages (from-to)415-423
Number of pages9
JournalJournal of Pain Research
Publication statusPublished - 2014 Jul 11


  • BDNF
  • Bone cancer pain
  • Chronic pain
  • Nerve growth factor


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