Use of native gels to measure protein binding to SSB

Jin Inoue, Tsutomu Mikawa

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

Original languageEnglish
Title of host publicationSingle-Stranded DNA Binding Proteins
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages8
ISBN (Print)9781627030311
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Electrophoresis
  • Mutational analysis
  • Native-PAGE
  • Protein-protein interaction
  • RecO
  • RecR
  • SSB-binding protein


Dive into the research topics of 'Use of native gels to measure protein binding to SSB'. Together they form a unique fingerprint.

Cite this