Use of native gels to measure protein binding to SSB

Jin Inoue; Tsutomu Mikawa

doi:10.1007/978-1-62703-32-8_13

Use of native gels to measure protein binding to SSB

Jin Inoue, Tsutomu Mikawa

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

Original language	English
Title of host publication	Single-Stranded DNA Binding Proteins
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	175-182
Number of pages	8
ISBN (Print)	9781627030311
DOIs	https://doi.org/10.1007/978-1-62703-32-8_13
Publication status	Published - 2012 Jan 1
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	922
ISSN (Print)	1064-3745

Keywords

Electrophoresis
Mutational analysis
Native-PAGE
Protein-protein interaction
RecO
RecR
SSB-binding protein

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-32-8_13

Cite this

@inbook{342afc30607b4b17a328403ff24d44ef,

title = "Use of native gels to measure protein binding to SSB",

abstract = "We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.",

keywords = "Electrophoresis, Mutational analysis, Native-PAGE, Protein-protein interaction, RecO, RecR, SSB-binding protein",

author = "Jin Inoue and Tsutomu Mikawa",

year = "2012",

month = jan,

day = "1",

doi = "10.1007/978-1-62703-32-8_13",

language = "English",

isbn = "9781627030311",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "175--182",

booktitle = "Single-Stranded DNA Binding Proteins",

}

TY - CHAP

T1 - Use of native gels to measure protein binding to SSB

AU - Inoue, Jin

AU - Mikawa, Tsutomu

PY - 2012/1/1

Y1 - 2012/1/1

N2 - We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

AB - We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.

KW - Electrophoresis

KW - Mutational analysis

KW - Native-PAGE

KW - Protein-protein interaction

KW - RecO

KW - RecR

KW - SSB-binding protein

UR - http://www.scopus.com/inward/record.url?scp=84934441958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934441958&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-32-8_13

DO - 10.1007/978-1-62703-32-8_13

M3 - Chapter

C2 - 22976186

AN - SCOPUS:84934441958

SN - 9781627030311

T3 - Methods in Molecular Biology

SP - 175

EP - 182

BT - Single-Stranded DNA Binding Proteins

PB - Humana Press Inc.

ER -

Use of native gels to measure protein binding to SSB

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this