Use of the avidin-biotin system for immobilization of an enzyme on the electrode surface

Jun ichi Anzai; Tomonori Hoshi; Shouryu Lee; Tetsuo Osa

doi:10.1016/0925-4005(93)85326-6

Use of the avidin-biotin system for immobilization of an enzyme on the electrode surface

Jun ichi Anzai, Tomonori Hoshi, Shouryu Lee, Tetsuo Osa

HOSP - Clinical Research, Innovation and Education Center

Tohoku University

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Two different procedures were developed for immobilizing an enzyme on the electrode surface by using the strong complexation of avidin and the biotinylated enzyme. The first method is based on the adsorption of avidin or a complex of avidin and the biotinylated enzyme on the LB film-coated electrode. The second procedure involves the direct electro-deposition of avidin or the avidin-biotinylated enzume complex. Response characteristics of glucose sensors prepared by both techniques were discussed in terms of the enzyme loading on the electrode surface.

Original language	English
Pages (from-to)	73-75
Number of pages	3
Journal	Sensors and Actuators B: Chemical
Volume	13
Issue number	1-3
DOIs	https://doi.org/10.1016/0925-4005(93)85326-6
Publication status	Published - 1993 May

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abstract = "Two different procedures were developed for immobilizing an enzyme on the electrode surface by using the strong complexation of avidin and the biotinylated enzyme. The first method is based on the adsorption of avidin or a complex of avidin and the biotinylated enzyme on the LB film-coated electrode. The second procedure involves the direct electro-deposition of avidin or the avidin-biotinylated enzume complex. Response characteristics of glucose sensors prepared by both techniques were discussed in terms of the enzyme loading on the electrode surface.",

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AU - Lee, Shouryu

AU - Osa, Tetsuo

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Use of the avidin-biotin system for immobilization of an enzyme on the electrode surface

Abstract

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