Variable gene segment-specific N-insertions at the signal joint of T- cell receptor Vβ-Dβ recombinations

Yasuyoshi Kanari; Ryusuke Nakagawa; Hiroshi Arakawa; Hideo Yamagishi

doi:10.1016/S0165-2478(98)00012-1

Variable gene segment-specific N-insertions at the signal joint of T- cell receptor Vβ-Dβ recombinations

Yasuyoshi Kanari, Ryusuke Nakagawa, Hiroshi Arakawa, Hideo Yamagishi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vβ- Dβ recombinations. N-insertions were detected at signal joints of Vβ2, Vβ3, Vβ10, Vβ18 and Vβ14 but not in Vβ8 and Vβ7. These data show that the N-insertion at signal joints is dependent on the Vβ locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.

Original language	English
Pages (from-to)	151-155
Number of pages	5
Journal	Immunology Letters
Volume	61
Issue number	2-3
DOIs	https://doi.org/10.1016/S0165-2478(98)00012-1
Publication status	Published - 1998 Apr
Externally published	Yes

Keywords

N-insertion
Signal joint
T-cell receptor β
V-DJ recombination

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0165-2478(98)00012-1

Cite this

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title = "Variable gene segment-specific N-insertions at the signal joint of T- cell receptor Vβ-Dβ recombinations",

abstract = "The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vβ- Dβ recombinations. N-insertions were detected at signal joints of Vβ2, Vβ3, Vβ10, Vβ18 and Vβ14 but not in Vβ8 and Vβ7. These data show that the N-insertion at signal joints is dependent on the Vβ locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.",

keywords = "N-insertion, Signal joint, T-cell receptor β, V-DJ recombination",

author = "Yasuyoshi Kanari and Ryusuke Nakagawa and Hiroshi Arakawa and Hideo Yamagishi",

note = "Funding Information: This work was supported by Grants-in-Aid for Science Research from the Ministry of Education, Science, Sports and Culture of Japan.",

year = "1998",

month = apr,

doi = "10.1016/S0165-2478(98)00012-1",

language = "English",

volume = "61",

pages = "151--155",

journal = "Immunology Letters",

issn = "0165-2478",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - Variable gene segment-specific N-insertions at the signal joint of T- cell receptor Vβ-Dβ recombinations

AU - Kanari, Yasuyoshi

AU - Nakagawa, Ryusuke

AU - Arakawa, Hiroshi

AU - Yamagishi, Hideo

N1 - Funding Information: This work was supported by Grants-in-Aid for Science Research from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 1998/4

Y1 - 1998/4

N2 - The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vβ- Dβ recombinations. N-insertions were detected at signal joints of Vβ2, Vβ3, Vβ10, Vβ18 and Vβ14 but not in Vβ8 and Vβ7. These data show that the N-insertion at signal joints is dependent on the Vβ locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.

AB - The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vβ- Dβ recombinations. N-insertions were detected at signal joints of Vβ2, Vβ3, Vβ10, Vβ18 and Vβ14 but not in Vβ8 and Vβ7. These data show that the N-insertion at signal joints is dependent on the Vβ locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.

KW - N-insertion

KW - Signal joint

KW - T-cell receptor β

KW - V-DJ recombination

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U2 - 10.1016/S0165-2478(98)00012-1

DO - 10.1016/S0165-2478(98)00012-1

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AN - SCOPUS:0032052230

SN - 0165-2478

VL - 61

SP - 151

EP - 155

JO - Immunology Letters

JF - Immunology Letters

IS - 2-3

ER -

Variable gene segment-specific N-insertions at the signal joint of T- cell receptor Vβ-Dβ recombinations

Abstract

Keywords

ASJC Scopus subject areas

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