Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3

Sadao Takahashi; Koji Oida; Minoru Ookubo; Jinya Suzuki; Mitsuyuki Kohno; Toshio Murase; Tokuo Yamamoto; Tsuguhiko Nakai

doi:10.1016/0014-5793(96)00439-5

Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3

Sadao Takahashi, Koji Oida, Minoru Ookubo, Jinya Suzuki, Mitsuyuki Kohno, Toshio Murase, Tokuo Yamamoto, Tsuguhiko Nakai

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.

Original language	English
Pages (from-to)	197-200
Number of pages	4
Journal	FEBS Letters
Volume	386
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)00439-5
Publication status	Published - 1996 May 20
Externally published	Yes

Keywords

Apolipoprotein E
Lipoprotein
Lipoprotein receptor
VLDL receptor
Very low density lipoprotein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(96)00439-5

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title = "Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3",

abstract = "The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.",

keywords = "Apolipoprotein E, Lipoprotein, Lipoprotein receptor, VLDL receptor, Very low density lipoprotein",

author = "Sadao Takahashi and Koji Oida and Minoru Ookubo and Jinya Suzuki and Mitsuyuki Kohno and Toshio Murase and Tokuo Yamamoto and Tsuguhiko Nakai",

note = "Funding Information: Acknowledgements: We thank Dr. Yves Marcel for providing monoclonal antibodies directed against apoE, 1D7 and 6C5, Dr. Monty Krieger for providing ldlA-7 cells, Drs. Yutaka Kawarabayasi and Juro Sakai for helpful discussion and Naoyo Yamaguchi for technical assistance. This work was supported in part by grants-in-aid for Scientific Research from the Ministry of Science, Education, and Culture of Japan, the Ministry of Health and Welfare.",

year = "1996",

month = may,

day = "20",

doi = "10.1016/0014-5793(96)00439-5",

language = "English",

volume = "386",

pages = "197--200",

journal = "FEBS Letters",

issn = "0014-5793",

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TY - JOUR

T1 - Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3

AU - Takahashi, Sadao

AU - Oida, Koji

AU - Ookubo, Minoru

AU - Suzuki, Jinya

AU - Kohno, Mitsuyuki

AU - Murase, Toshio

AU - Yamamoto, Tokuo

AU - Nakai, Tsuguhiko

N1 - Funding Information: Acknowledgements: We thank Dr. Yves Marcel for providing monoclonal antibodies directed against apoE, 1D7 and 6C5, Dr. Monty Krieger for providing ldlA-7 cells, Drs. Yutaka Kawarabayasi and Juro Sakai for helpful discussion and Naoyo Yamaguchi for technical assistance. This work was supported in part by grants-in-aid for Scientific Research from the Ministry of Science, Education, and Culture of Japan, the Ministry of Health and Welfare.

PY - 1996/5/20

Y1 - 1996/5/20

N2 - The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.

AB - The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.

KW - Apolipoprotein E

KW - Lipoprotein

KW - Lipoprotein receptor

KW - VLDL receptor

KW - Very low density lipoprotein

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ER -

Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3

Abstract

Keywords

ASJC Scopus subject areas

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