Visualization of microorganisms in bioprocesses

Tsuyoshi Yamaguchi; Kengo Kubota

doi:10.1007/978-981-10-6863-8_2

Visualization of microorganisms in bioprocesses

Tsuyoshi Yamaguchi, Kengo Kubota

ENG - Civil and Environmental Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) is currently a standard technique for detecting, identifying, and enumerating microorganisms. The method allows for visualization of cell morphology as well as in situ localization of microorganisms at single-cell resolution. Although FISH has been used for more than 20 years, many challenges remain with regard to improving the technique for understanding microbial ecology and physiology. This chapter describes the recent developments in this field, such as improved sensitivity by catalyzed reporter deposition (CARD)-FISH and in situ DNA-hybridization chain reaction (HCR). Highly sensitive methods for detecting mRNA and/or functional genes are also described. Moreover, methods combining isotope probing to reveal microbial metabolic activities are introduced.

Original language	English
Title of host publication	Optimization and Applicability of Bioprocesses
Publisher	Springer Singapore
Pages	13-26
Number of pages	14
ISBN (Electronic)	9789811068638
ISBN (Print)	9789811068621
DOIs	https://doi.org/10.1007/978-981-10-6863-8_2
Publication status	Published - 2018 Jan 2

Keywords

Ecology
Fluorescence
Metabolic activity
Microorganisms
Phylogeny

ASJC Scopus subject areas

Medicine(all)
Health Professions(all)
Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)
Environmental Science(all)
Immunology and Microbiology(all)

Access to Document

10.1007/978-981-10-6863-8_2

Cite this

@inbook{d18ea1d269ce40118035f957908bd44d,

title = "Visualization of microorganisms in bioprocesses",

abstract = "Fluorescence in situ hybridization (FISH) is currently a standard technique for detecting, identifying, and enumerating microorganisms. The method allows for visualization of cell morphology as well as in situ localization of microorganisms at single-cell resolution. Although FISH has been used for more than 20 years, many challenges remain with regard to improving the technique for understanding microbial ecology and physiology. This chapter describes the recent developments in this field, such as improved sensitivity by catalyzed reporter deposition (CARD)-FISH and in situ DNA-hybridization chain reaction (HCR). Highly sensitive methods for detecting mRNA and/or functional genes are also described. Moreover, methods combining isotope probing to reveal microbial metabolic activities are introduced.",

keywords = "Ecology, Fluorescence, Metabolic activity, Microorganisms, Phylogeny",

author = "Tsuyoshi Yamaguchi and Kengo Kubota",

year = "2018",

month = jan,

day = "2",

doi = "10.1007/978-981-10-6863-8_2",

language = "English",

isbn = "9789811068621",

pages = "13--26",

booktitle = "Optimization and Applicability of Bioprocesses",

publisher = "Springer Singapore",

}

TY - CHAP

T1 - Visualization of microorganisms in bioprocesses

AU - Yamaguchi, Tsuyoshi

AU - Kubota, Kengo

PY - 2018/1/2

Y1 - 2018/1/2

N2 - Fluorescence in situ hybridization (FISH) is currently a standard technique for detecting, identifying, and enumerating microorganisms. The method allows for visualization of cell morphology as well as in situ localization of microorganisms at single-cell resolution. Although FISH has been used for more than 20 years, many challenges remain with regard to improving the technique for understanding microbial ecology and physiology. This chapter describes the recent developments in this field, such as improved sensitivity by catalyzed reporter deposition (CARD)-FISH and in situ DNA-hybridization chain reaction (HCR). Highly sensitive methods for detecting mRNA and/or functional genes are also described. Moreover, methods combining isotope probing to reveal microbial metabolic activities are introduced.

AB - Fluorescence in situ hybridization (FISH) is currently a standard technique for detecting, identifying, and enumerating microorganisms. The method allows for visualization of cell morphology as well as in situ localization of microorganisms at single-cell resolution. Although FISH has been used for more than 20 years, many challenges remain with regard to improving the technique for understanding microbial ecology and physiology. This chapter describes the recent developments in this field, such as improved sensitivity by catalyzed reporter deposition (CARD)-FISH and in situ DNA-hybridization chain reaction (HCR). Highly sensitive methods for detecting mRNA and/or functional genes are also described. Moreover, methods combining isotope probing to reveal microbial metabolic activities are introduced.

KW - Ecology

KW - Fluorescence

KW - Metabolic activity

KW - Microorganisms

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=85046026620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046026620&partnerID=8YFLogxK

U2 - 10.1007/978-981-10-6863-8_2

DO - 10.1007/978-981-10-6863-8_2

M3 - Chapter

AN - SCOPUS:85046026620

SN - 9789811068621

SP - 13

EP - 26

BT - Optimization and Applicability of Bioprocesses

PB - Springer Singapore

ER -

Visualization of microorganisms in bioprocesses

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this