TY - JOUR
T1 - Visualizing Intramolecular Dynamics of Membrane Proteins
AU - Ohkubo, Tatsunari
AU - Shiina, Takaaki
AU - Kawaguchi, Kayoko
AU - Sasaki, Daisuke
AU - Inamasu, Rena
AU - Yang, Yue
AU - Li, Zhuoqi
AU - Taninaka, Keizaburo
AU - Sakaguchi, Masaki
AU - Fujimura, Shoko
AU - Sekiguchi, Hiroshi
AU - Kuramochi, Masahiro
AU - Arai, Tatsuya
AU - Tsuda, Sakae
AU - Sasaki, Yuji C.
AU - Mio, Kazuhiro
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented.
AB - Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented.
KW - conformation dynamics
KW - diffracted X-ray tracking technique
KW - membrane proteins
KW - single-molecule analysis
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U2 - 10.3390/ijms232314539
DO - 10.3390/ijms232314539
M3 - Review article
C2 - 36498865
AN - SCOPUS:85143689013
SN - 1661-6596
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 23
M1 - 14539
ER -