Visualizing Intramolecular Dynamics of Membrane Proteins

Tatsunari Ohkubo, Takaaki Shiina, Kayoko Kawaguchi, Daisuke Sasaki, Rena Inamasu, Yue Yang, Zhuoqi Li, Keizaburo Taninaka, Masaki Sakaguchi, Shoko Fujimura, Hiroshi Sekiguchi, Masahiro Kuramochi, Tatsuya Arai, Sakae Tsuda, Yuji C. Sasaki, Kazuhiro Mio

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)

Abstract

Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented.

Original languageEnglish
Article number14539
JournalInternational Journal of Molecular Sciences
Volume23
Issue number23
DOIs
Publication statusPublished - 2022 Dec

Keywords

  • conformation dynamics
  • diffracted X-ray tracking technique
  • membrane proteins
  • single-molecule analysis

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