Vitamin D upregulates expression of ECaC1 mRNA in semicircular canal

Daisuke Yamauchi, Nithya N. Raveendran, Satyanarayana R. Pondugula, Suresh B. Kampalli, Joel D. Sanneman, Donald G. Harbidge, Daniel C. Marcus

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57 Citations (Scopus)


The low luminal Ca2+ concentration of mammalian endolymph in the vestibular labyrinth is required for normal balance. We found transcripts in primary cultures of semicircular canal duct (SCCD) epithelial cells from neonatal rats representing a complete transport system for transepithelial absorption of Ca2+ that is comprised of the epithelial Ca 2+ channels ECaC1 (CaT2, TRPV5) and ECaC2 (CaT1, TRPV6), calbindin (calbindin-D9k, calbindin-D28k), Na+/Ca2+ exchanger (NCX1, NCX2, and NCX3), and plasma membrane Ca2+-ATPase (PMCA1, PMCA3, and PMCA4) by RT-PCR. Further, vitamin D receptor was also expressed in SCCD and it was found by quantitative RT-PCR that incubation for 24 h with 1,25-dihydroxyvitamin D3 upregulated the expression of ECaC1, calbindin-D9k, and calbindin-D28k. These observations provide evidence for the first time of an ECaC-based Ca2+ transport system in SCCD that could maintain the low Ca2+ concentration in vestibular endolymph.

Original languageEnglish
Pages (from-to)1353-1357
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number4
Publication statusPublished - 2005 Jun 17


  • 1,25-Dihydroxyvitamin D
  • Calbindin
  • Epithelial calcium channel
  • Na /Ca exchanger
  • Plasma membrane Ca-ATPase
  • Semicircular canal duct
  • Vitamin D receptor


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