WFS1 protein modulates the free Ca2+ concentration in the endoplasmic reticulum

Daisuke Takei, Hisamitsu Ishihara, Suguru Yamaguchi, Takahiro Yamada, Akira Tamura, Hideki Katagiri, Yoshio Maruyama, Yoshitomo Oka

Research output: Contribution to journalArticlepeer-review

120 Citations (Scopus)


The WFS1 gene, encoding an endoplasmic reticulum (ER) membrane glycoprotein, is mutated in Wolfram syndrome characterized by diabetes mellitus and optic atrophy. Herein, Ca2+ dynamics were examined in WFS1-knockdown and -overexpressing HEK293 cells. Studies using ER-targeted Ca2+-sensitive photoprotein aequorin demonstrated WFS1 protein to positively modulate ER Ca2+ levels by increasing the rate of Ca2+ uptake. Furthermore, Ca2+ imaging with Fura-2 showed the magnitude of the store-operated Ca2+ entry to parallel WFS1 expression levels. These data indicate that WFS1 protein participates in the regulation of cellular Ca2+ homeostasis, at least partly, by modulating the filling state of the ER Ca2+ store.

Original languageEnglish
Pages (from-to)5635-5640
Number of pages6
JournalFEBS Letters
Issue number24
Publication statusPublished - 2006 Oct 16


  • Cellular Ca homeostasis
  • Endoplasmic reticulum
  • WFS1
  • Wolfram syndrome

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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