WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The gene RESISTANCE TO CMV(Y) (RCY1) encodes a coiled-coil (CC) nucleotide-binding (NB) leucine-rich repeat (LRR)-class resistance protein that confers resistance to a yellow strain of Cucumber mosaic virus [CMV(Y)] with developing the hypersensitive response (HR) cell death in virus-inoculated leaves of Arabidopsis thaliana. To understand the RCY1-mediated virus defense response, host protein that interacts with the CC and NB domains of RCY1 was isolated in a yeast two-hybrid system using the CC-NB domain fragment as bait. The WRKY70 transcription factor could reproducibly bind to the CC-NB domain fragment. This interaction between WRKY70 and the CC-NB domains was confirmed in a co-immunopurification experiment, suggesting that WRKY70 may function in RCY1-conferred resistance to CMV(Y). In CMV(Y)-inoculated leaves of wild-type plants carrying RCY1, virus multiplication was suppressed, and the level of WRKY70 and Pathogenesis-Related 1 (PR-1) transcripts clearly increased. On the other hand, in CMV(Y)-inoculated leaves of the RCY1-transformed wrky70-1 mutant lacking WRKY70 transcripts, virus coat protein accumulation increased significantly, and the induction of PR-1 expression in response to CMV(Y) was partially compromised. However, there was no significant difference in the degree of HR cell death between the wrky70-1 and wild-type plants carrying RCY1. These results indicate that WRKY70 may play a critical role in suppressing virus multiplication, but not in elaborating HR cell death, by directly interacting with the CC-NB domains of RCY1.

Original languageEnglish
Pages (from-to)8-14
Number of pages7
JournalPhysiological and Molecular Plant Pathology
Volume85
DOIs
Publication statusPublished - 2014 Jan

Keywords

  • Cucumber mosaic virus
  • Hypersensitive response
  • NB-LRR protein
  • Virus resistance gene
  • WRKY transcription factor

Fingerprint

Dive into the research topics of 'WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this