TY - JOUR
T1 - A new role for histone demethylases in the maintenance of plant genome integrity
AU - Antunez-Sanchez, Javier
AU - Naish, Matthew
AU - Ramirez-Prado, Juan Sebastian
AU - Ohno, Sho
AU - Huang, Ying
AU - Dawson, Alexander
AU - Opassathian, Korawit
AU - Manza-Mianza, Deborah
AU - Ariel, Federico
AU - Raynaud, Cecile
AU - Wibowo, Anjar
AU - Daron, Josquin
AU - Ueda, Minako
AU - Latrasse, David
AU - Slotkin, R. Keith
AU - Weigel, Detlef
AU - Benhamed, Moussa
AU - Gutierrez-Marcos, Jose
N1 - Funding Information:
We thank Gary Grant for help with plant husbandry; Xiaofeng Cao and Caroline Dean for seed stocks and data. Ranjth Papareddy for the identification of ref6-5 and Liliana M. Costa for discussions and comments on the manuscript. Supported by ERA-CAPS Program (Project AUREATE), and the Max Planck Society (D.W.), ANR/CNRS grant (EpiGEN) to M.B., JSPS grant (JP19H05676) to M.U. and BBSRC grants (BB/L003023/1, BB/N005279/1, BB/N00194X/1 and BB/P02601X/1) to J.G-M.
Publisher Copyright:
© 2020, eLife Sciences Publications Ltd. All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in the control of growth, development, and adaptation to environmental fluctuations of most multicellular eukaryotes. The catalytic activity of PRC2 is counteracted by Jumonji-type (JMJ) histone demethylases, which shapes the genomic distribution of H3K27me3. Here, we show that two JMJ histone demethylases in Arabidopsis, EARLY FLOWERING 6 (ELF6) and RELATIVE OF EARLY FLOWERING 6 (REF6), play distinct roles in H3K27me3 and H3K27me1 homeostasis. We show that failure to reset these chromatin marks during sexual reproduction results in the transgenerational inheritance of histone marks, which cause a loss of DNA methylation at heterochromatic loci and transposon activation. Thus, Jumonji-type histone demethylases play a dual role in plants by helping to maintain transcriptional states through development and safeguard genome integrity during sexual reproduction.
AB - Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in the control of growth, development, and adaptation to environmental fluctuations of most multicellular eukaryotes. The catalytic activity of PRC2 is counteracted by Jumonji-type (JMJ) histone demethylases, which shapes the genomic distribution of H3K27me3. Here, we show that two JMJ histone demethylases in Arabidopsis, EARLY FLOWERING 6 (ELF6) and RELATIVE OF EARLY FLOWERING 6 (REF6), play distinct roles in H3K27me3 and H3K27me1 homeostasis. We show that failure to reset these chromatin marks during sexual reproduction results in the transgenerational inheritance of histone marks, which cause a loss of DNA methylation at heterochromatic loci and transposon activation. Thus, Jumonji-type histone demethylases play a dual role in plants by helping to maintain transcriptional states through development and safeguard genome integrity during sexual reproduction.
KW - Arabidopsis
KW - Chromatin
KW - DNA methylation
KW - Epimutation
KW - Transposon
UR - http://www.scopus.com/inward/record.url?scp=85096357188&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096357188&partnerID=8YFLogxK
U2 - 10.7554/eLife.58533
DO - 10.7554/eLife.58533
M3 - Article
C2 - 33107825
AN - SCOPUS:85096357188
SN - 2050-084X
VL - 9
SP - 1
EP - 32
JO - eLife
JF - eLife
M1 - e58533
ER -