@article{a8003ef25e864578ac560b911ac64637,
title = "Affinity purification and characterization of a key enzyme responsible for circadian rhythmic control of nyctinasty in Lespedeza cuneata L",
abstract = "The synthesis of an affinity gel aimed at leaf-opening factor β-glucosidase (LOFG) and affinity purification of LOFG is presented. A gluconamidine-based β-glucosidase inhibitor was used as the ligand of the affinity gel. β-Glucosidase exhibiting an activity shift throughout the day was selectively purified from Lespedeza cuneata Don by the affinity gel. The resulting LOFG exhibited high substrate specificity toward the leaf-opening factor.",
keywords = "Affinity purification, Gluconamidine, Inhibitor, Nyctinasty, β-Glucosidase",
author = "Eisuke Kato and Takehiko Sasaki and Minoru Ueda",
note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research on Priority Area 16073216 from the Ministry of Education, Science, Sports, and Culture (MEXT), Asahi Glass Foundation, and Naito Foundation. We thank Assoc. Prof. Jun Hiratake and Prof. Kanzo Sakata (Institute for Chemical Research, Kyoto University) for the helpful discussions and suggestions on this work.",
year = "2008",
month = apr,
day = "15",
doi = "10.1016/j.bmc.2008.02.035",
language = "English",
volume = "16",
pages = "4600--4616",
journal = "Bioorganic and Medicinal Chemistry",
issn = "0968-0896",
publisher = "Elsevier Limited",
number = "8",
}