AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle

Masato Miyake; Hideyuki Takahashi; Eri Kitagawa; Hitoshi Watanabe; Takahiro Sakurada; Hisashi Aso; Takahiro Yamaguchi

doi:10.1007/s00441-012-1422-8

AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle

Masato Miyake, Hideyuki Takahashi, Eri Kitagawa, Hitoshi Watanabe, Takahiro Sakurada, Hisashi Aso, Takahiro Yamaguchi

研究成果: Article › 査読

36 被引用数 (Scopus)

抄録

AMP-activated protein kinase (AMPK) regulates metabolism in skeletal muscle and myostatin (MSTN) negatively regulates skeletal muscle development and growth. In the present study, AMPK activation and the relationship between AMPK and MSTN during myogenic differentiation were investigated in cultures derived from bovine skeletal muscle. Myoblasts capable of forming myotubes were obtained from bovine skeletal muscle and treated with AICAR to activate AMPK, resulting in suppressed myotube formation. AICAR treatment significantly reduced the expression of MSTN mRNA during myogenic differentiation. Combined treatment with AICAR and MSTN suppressed myotube formation to a greater extent than AICAR alone. SB431542, an inhibitor of MSTN signaling, promoted myotube formation during myogenic differentiation. However, simultaneous treatment with AICAR blocked this effect of SB431542. Therefore, AMPK activation inhibits myogenic differentiation but may suppress MSTN expression to balance muscle development.

本文言語	English
ページ（範囲）	615-623
ページ数	9
ジャーナル	Cell and Tissue Research
巻	349
号	2
DOI	https://doi.org/10.1007/s00441-012-1422-8
出版ステータス	Published - 2012 8月

ASJC Scopus subject areas

病理学および法医学
組織学
細胞生物学

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10.1007/s00441-012-1422-8

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title = "AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle",

abstract = "AMP-activated protein kinase (AMPK) regulates metabolism in skeletal muscle and myostatin (MSTN) negatively regulates skeletal muscle development and growth. In the present study, AMPK activation and the relationship between AMPK and MSTN during myogenic differentiation were investigated in cultures derived from bovine skeletal muscle. Myoblasts capable of forming myotubes were obtained from bovine skeletal muscle and treated with AICAR to activate AMPK, resulting in suppressed myotube formation. AICAR treatment significantly reduced the expression of MSTN mRNA during myogenic differentiation. Combined treatment with AICAR and MSTN suppressed myotube formation to a greater extent than AICAR alone. SB431542, an inhibitor of MSTN signaling, promoted myotube formation during myogenic differentiation. However, simultaneous treatment with AICAR blocked this effect of SB431542. Therefore, AMPK activation inhibits myogenic differentiation but may suppress MSTN expression to balance muscle development.",

keywords = "AMPK, Cattle, Differentiation, Myoblast, Myostatin",

author = "Masato Miyake and Hideyuki Takahashi and Eri Kitagawa and Hitoshi Watanabe and Takahiro Sakurada and Hisashi Aso and Takahiro Yamaguchi",

note = "Funding Information: Acknowledgment This work was supported by a Grant-in-Aid for Scientific Research (22880006) from the Ministry of Education, Culture, Sports, Science and Technology (to M.M) and the Programme for Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry (to T.Y). This paper is dedicated to Professor Takahiro Yamaguchi who passed away during completion of this work.",

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T1 - AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle

AU - Miyake, Masato

AU - Takahashi, Hideyuki

AU - Kitagawa, Eri

AU - Watanabe, Hitoshi

AU - Sakurada, Takahiro

AU - Aso, Hisashi

AU - Yamaguchi, Takahiro

N1 - Funding Information: Acknowledgment This work was supported by a Grant-in-Aid for Scientific Research (22880006) from the Ministry of Education, Culture, Sports, Science and Technology (to M.M) and the Programme for Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry (to T.Y). This paper is dedicated to Professor Takahiro Yamaguchi who passed away during completion of this work.

PY - 2012/8

Y1 - 2012/8

N2 - AMP-activated protein kinase (AMPK) regulates metabolism in skeletal muscle and myostatin (MSTN) negatively regulates skeletal muscle development and growth. In the present study, AMPK activation and the relationship between AMPK and MSTN during myogenic differentiation were investigated in cultures derived from bovine skeletal muscle. Myoblasts capable of forming myotubes were obtained from bovine skeletal muscle and treated with AICAR to activate AMPK, resulting in suppressed myotube formation. AICAR treatment significantly reduced the expression of MSTN mRNA during myogenic differentiation. Combined treatment with AICAR and MSTN suppressed myotube formation to a greater extent than AICAR alone. SB431542, an inhibitor of MSTN signaling, promoted myotube formation during myogenic differentiation. However, simultaneous treatment with AICAR blocked this effect of SB431542. Therefore, AMPK activation inhibits myogenic differentiation but may suppress MSTN expression to balance muscle development.

AB - AMP-activated protein kinase (AMPK) regulates metabolism in skeletal muscle and myostatin (MSTN) negatively regulates skeletal muscle development and growth. In the present study, AMPK activation and the relationship between AMPK and MSTN during myogenic differentiation were investigated in cultures derived from bovine skeletal muscle. Myoblasts capable of forming myotubes were obtained from bovine skeletal muscle and treated with AICAR to activate AMPK, resulting in suppressed myotube formation. AICAR treatment significantly reduced the expression of MSTN mRNA during myogenic differentiation. Combined treatment with AICAR and MSTN suppressed myotube formation to a greater extent than AICAR alone. SB431542, an inhibitor of MSTN signaling, promoted myotube formation during myogenic differentiation. However, simultaneous treatment with AICAR blocked this effect of SB431542. Therefore, AMPK activation inhibits myogenic differentiation but may suppress MSTN expression to balance muscle development.

KW - AMPK

KW - Cattle

KW - Differentiation

KW - Myoblast

KW - Myostatin

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AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle

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