TY - JOUR
T1 - Antibacterial activity of Microbispora rosea subsp. rosea SL3-2-R-1 grown on different media and solidifying agents
AU - Ningsih, F.
AU - Sari, D. C.A.F.
AU - Yabe, S.
AU - Yokota, A.
AU - Oetari, A.
AU - Sjamsuridzal, W.
N1 - Funding Information:
This study was supported by Hibah Publikasi Terindeks Internasional (PUTI) Prosiding Tahun Anggaran 2020 Universitas Indonesia contract number: NKB-3537/UN2.RST/HKP.05.00/2020 to W.S.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/6/14
Y1 - 2021/6/14
N2 - The aim of this study was to examine the effect of different media and solidifying agents as growth media of a rare thermophilic actinobacterium, strain Microbispora rosea subsp. rosea SL3-2-R-1, on its antibacterial activity. To investigate the antibacterial potential, the strain was grown on four media, e.g., International Streptomyces Project (ISP) 1, ISP 2, ISP 3, and Bennett's medium, solidified with agar and gellan gum, at 45 °C for 21-days. The antibacterial activity screening was performed using the agar plug diffusion method against four bacterial test strains (S. aureus, B. subtilis, K. rhizophila, and E. coli) grown on Mueller-Hinton agar, incubated at 30 °C for 17 h. The antibacterial activity was observed on strain grown on ISP 1 gellan gum, ISP 2 gellan gum, ISP 3 agar, ISP 3 gellan gum, and Bennett's agar against B. subtilis. The strain grown on ISP 1 gellan gum, ISP 3 agar, and ISP 3 gellan gum inhibited the growth of K. rhizophila, while on ISP 1, gellan gum and ISP 3 agar were positive against S. aureus. However, on all media, the strain showed no inhibition against E. coli.
AB - The aim of this study was to examine the effect of different media and solidifying agents as growth media of a rare thermophilic actinobacterium, strain Microbispora rosea subsp. rosea SL3-2-R-1, on its antibacterial activity. To investigate the antibacterial potential, the strain was grown on four media, e.g., International Streptomyces Project (ISP) 1, ISP 2, ISP 3, and Bennett's medium, solidified with agar and gellan gum, at 45 °C for 21-days. The antibacterial activity screening was performed using the agar plug diffusion method against four bacterial test strains (S. aureus, B. subtilis, K. rhizophila, and E. coli) grown on Mueller-Hinton agar, incubated at 30 °C for 17 h. The antibacterial activity was observed on strain grown on ISP 1 gellan gum, ISP 2 gellan gum, ISP 3 agar, ISP 3 gellan gum, and Bennett's agar against B. subtilis. The strain grown on ISP 1 gellan gum, ISP 3 agar, and ISP 3 gellan gum inhibited the growth of K. rhizophila, while on ISP 1, gellan gum and ISP 3 agar were positive against S. aureus. However, on all media, the strain showed no inhibition against E. coli.
UR - http://www.scopus.com/inward/record.url?scp=85108580659&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85108580659&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1918/5/052011
DO - 10.1088/1742-6596/1918/5/052011
M3 - Conference article
AN - SCOPUS:85108580659
SN - 1742-6588
VL - 1918
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 5
M1 - 052011
T2 - 7th International Conference on Mathematics, Science, and Education 2020, ICMSE 2020
Y2 - 6 October 2020 through 6 October 2020
ER -