Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

Takakazu Nakabayashi; Hui Ping Wang; Masataka Kinjo; Nobuhiro Ohta

doi:10.1039/b800391b

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

Takakazu Nakabayashi, Hui Ping Wang, Masataka Kinjo, Nobuhiro Ohta

研究成果: Article › 査読

62 被引用数 (Scopus)

抄録

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

本文言語	English
ページ（範囲）	668-670
ページ数	3
ジャーナル	Photochemical and Photobiological Sciences
巻	7
号	6
DOI	https://doi.org/10.1039/b800391b
出版ステータス	Published - 2008
外部発表	はい

ASJC Scopus subject areas

物理化学および理論化学

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10.1039/b800391b

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abstract = "We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.",

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AU - Kinjo, Masataka

AU - Ohta, Nobuhiro

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AB - We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

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Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

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