Biomechanics of C2C12 Cells Observed with Cellular Resolution Scanning Acoustic Microscope Combined with Optical Microscope

Ryo Hirano, Makoto Kanzaki, Mototaka Arakawa, Norma Hermawan, Kazuto Kobayashi, Yoshifumi Saijo

研究成果: 書籍の章/レポート/Proceedings会議への寄与査読

抄録

Biomechanics of the cell indicates the inner structure and viability of the cell. Mechanical properties are represented by acoustic properties such as speed of sound (SOS) or acoustic impedance. In the present study, cellular resolution scanning acoustic microscope combined with optical microscope (OptSAM) is developed to observe the change of mechanical properties in cell differentiation. Main part of the OptSAM was consisted of 350 MHz ultrasound transducer mechanically scanned by a piezo-actuator. Thickness, SOS, acoustic impedance, density and elastic bulk modulus of the cell were deduced by the ultrasound responses in both time domain and frequency domain. C2C12 cell changing its form from myoblast to myotube was observed by OptSAM. The value of bulk modulus slightly increased in response to differentiation process. OptSAM non-invasively provides important information on biomechanics of cells without contact or staining.

本文言語英語
ホスト出版物のタイトル2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
出版社Institute of Electrical and Electronics Engineers Inc.
ページ4828-4831
ページ数4
ISBN(電子版)9781538613115
DOI
出版ステータス出版済み - 2019 7月
イベント41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 - Berlin, ドイツ
継続期間: 2019 7月 232019 7月 27

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

会議

会議41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
国/地域ドイツ
CityBerlin
Period19/7/2319/7/27

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