Cell collecting device by micro vibration and cavitation generated by ultrasonic irradiation

In this study, a device to collect cells in extreme minimally invasive way by using ultrasound was designed and fabricated. Change in amount of proteins RNA in living cells has vast amount of different information that enable us to understand an individual's health condition. Dynamic cellular activities can be revealed by analyzing living cells at different points of time in the day. In the fabricated device, ultrasound generated from a Langevin transducer is amplified by an ultrasonic horn and transmitted to a fine needle. Ultrasound is irradiated from the needle to body surface and ultrasonic oscillation and cavitation weaken the bond between the cells which were tightly connected, and the cells were aspirated. Using the excised skin tissue of pigs, cell aspiration experiments were performed at different frequencies and intensities of ultrasonic waves, at different extraction speed and time, thus suitable collection conditions were examined.